Abstract
An automated image analyzer was used to count lactate dehydrogenase positive alveolar wall cells (type 2 pneumocytes) in the lungs of a group of control guinea pigs and guinea pigs exposed to 2 ppm of nitrogen dioxide. The results from each of 141 photographic slides of the microscopic fields were compared with hand counts derived from a previous study of the same slides. A high coefficient of correlation was obtained (r >0.8; P<10-6), and the time required for counting was greatly reduced from several wk to less than 1 hr. The analysis, in conjunction with counts of alveoli, is a potential means of expediting and amplifying the quantitation of hyperplasia of type 2 pneumocytes secondary to a variety of noxious insults to the lung.
Original language | English (US) |
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Pages (from-to) | 704-707 |
Number of pages | 4 |
Journal | AMER.REV.RESP.DIS. |
Volume | 108 |
Issue number | 3 |
State | Published - 1973 |
Externally published | Yes |
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine