An image analyzer quantitation of type 2 pneumocytes

J. B. Margolick, S. P. Azen, R. P. Sherwin

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


An automated image analyzer was used to count lactate dehydrogenase positive alveolar wall cells (type 2 pneumocytes) in the lungs of a group of control guinea pigs and guinea pigs exposed to 2 ppm of nitrogen dioxide. The results from each of 141 photographic slides of the microscopic fields were compared with hand counts derived from a previous study of the same slides. A high coefficient of correlation was obtained (r >0.8; P<10-6), and the time required for counting was greatly reduced from several wk to less than 1 hr. The analysis, in conjunction with counts of alveoli, is a potential means of expediting and amplifying the quantitation of hyperplasia of type 2 pneumocytes secondary to a variety of noxious insults to the lung.

Original languageEnglish (US)
Pages (from-to)704-707
Number of pages4
Issue number3
StatePublished - 1973
Externally publishedYes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine


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