An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus

Chuan Qin; Jianwei Wang; Qiang Wei; Mingpeng She; Wayne A. Marasco; Hong Jiang; Xinming Tu; Hua Zhu; Lili Ren; Li Gao; Li Guo; Lan Huang; Renquan Yang; Zhe Cong; Lan Guo; Yanbin Wang; Yali Liu; Lili Sun; Shumin Duan; Jianguo Qu; Liangbiao Chen; Wei Tong; Li Ruan; Peimao Liu; Hua Zhang; Jianmin Zhang; Huiyuan Zhang; Depei Liu; Qian Liu; Tao Hong; Wei He

doi:10.1002/path.1769

An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus

Chuan Qin, Jianwei Wang, Qiang Wei, Mingpeng She, Wayne A. Marasco, Hong Jiang, Xinming Tu, Hua Zhu, Lili Ren, Li Gao, Li Guo, Lan Huang, Renquan Yang, Zhe Cong, Lan Guo, Yanbin Wang, Yali Liu, Lili Sun, Shumin Duan, Jianguo QuLiangbiao Chen, Wei Tong, Li Ruan, Peimao Liu, Hua Zhang, Jianmin Zhang, Huiyuan Zhang, Depei Liu, Qian Liu, Tao Hong, Wei He

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

A new SARS animal model was established by inoculating SARS coronavirus (SARS-CoV) into rhesus macaques (Macaca mulatta) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5-60 after virus inoculation. All eight animals showed a transient fever 2-3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS-CoV-specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS-CoV RNA could be detected in pharyngeal swab samples using nested RT-PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS-CoV-related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS.

Original language	English (US)
Pages (from-to)	251-259
Number of pages	9
Journal	Journal of Pathology
Volume	206
Issue number	3
DOIs	https://doi.org/10.1002/path.1769
State	Published - Jul 2005
Externally published	Yes

Keywords

Animal model
Coronavirus
Pathology
Rhesus monkey
SARS

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1002/path.1769

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Qin, C, Wang, J, Wei, Q, She, M, Marasco, WA, Jiang, H, Tu, X, Zhu, H, Ren, L, Gao, L, Guo, L, Huang, L, Yang, R, Cong, Z, Guo, L, Wang, Y, Liu, Y, Sun, L, Duan, S, Qu, J, Chen, L, Tong, W, Ruan, L, Liu, P, Zhang, H, Zhang, J, Zhang, H, Liu, D, Liu, Q, Hong, T & He, W 2005, 'An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus', Journal of Pathology, vol. 206, no. 3, pp. 251-259. https://doi.org/10.1002/path.1769

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title = "An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus",

abstract = "A new SARS animal model was established by inoculating SARS coronavirus (SARS-CoV) into rhesus macaques (Macaca mulatta) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5-60 after virus inoculation. All eight animals showed a transient fever 2-3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS-CoV-specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS-CoV RNA could be detected in pharyngeal swab samples using nested RT-PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS-CoV-related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS.",

keywords = "Animal model, Coronavirus, Pathology, Rhesus monkey, SARS",

author = "Chuan Qin and Jianwei Wang and Qiang Wei and Mingpeng She and Marasco, {Wayne A.} and Hong Jiang and Xinming Tu and Hua Zhu and Lili Ren and Li Gao and Li Guo and Lan Huang and Renquan Yang and Zhe Cong and Lan Guo and Yanbin Wang and Yali Liu and Lili Sun and Shumin Duan and Jianguo Qu and Liangbiao Chen and Wei Tong and Li Ruan and Peimao Liu and Hua Zhang and Jianmin Zhang and Huiyuan Zhang and Depei Liu and Qian Liu and Tao Hong and Wei He",

year = "2005",

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doi = "10.1002/path.1769",

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T1 - An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus

AU - Qin, Chuan

AU - Wang, Jianwei

AU - Wei, Qiang

AU - She, Mingpeng

AU - Marasco, Wayne A.

AU - Jiang, Hong

AU - Tu, Xinming

AU - Zhu, Hua

AU - Ren, Lili

AU - Gao, Li

AU - Guo, Li

AU - Huang, Lan

AU - Yang, Renquan

AU - Cong, Zhe

AU - Guo, Lan

AU - Wang, Yanbin

AU - Liu, Yali

AU - Sun, Lili

AU - Duan, Shumin

AU - Qu, Jianguo

AU - Chen, Liangbiao

AU - Tong, Wei

AU - Ruan, Li

AU - Liu, Peimao

AU - Zhang, Hua

AU - Zhang, Jianmin

AU - Zhang, Huiyuan

AU - Liu, Depei

AU - Liu, Qian

AU - Hong, Tao

AU - He, Wei

PY - 2005/7

Y1 - 2005/7

N2 - A new SARS animal model was established by inoculating SARS coronavirus (SARS-CoV) into rhesus macaques (Macaca mulatta) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5-60 after virus inoculation. All eight animals showed a transient fever 2-3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS-CoV-specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS-CoV RNA could be detected in pharyngeal swab samples using nested RT-PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS-CoV-related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS.

AB - A new SARS animal model was established by inoculating SARS coronavirus (SARS-CoV) into rhesus macaques (Macaca mulatta) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5-60 after virus inoculation. All eight animals showed a transient fever 2-3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS-CoV-specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS-CoV RNA could be detected in pharyngeal swab samples using nested RT-PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS-CoV-related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS.

KW - Animal model

KW - Coronavirus

KW - Pathology

KW - Rhesus monkey

KW - SARS

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An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus

Abstract

Keywords

ASJC Scopus subject areas

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