TY - JOUR
T1 - An alcian blue (ph 2.5)-pas-keratin immunoperoxidase method for the simultaneous demonstration of keratin and neutral and acidic mucosubstances
AU - Wilson, Thomas S.
AU - Mcdowell, Elizabeth M.
AU - Trump, Benjamin F.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - A method is proposed for the simultaneous staining of neutral and acidic, periodate reactive and nonperiodate reactive mucosubstances, glycogen and keratin in paraffin sections. Briefly, sections are stained by the Alcian blue (pH 2.5)-PAS method, followed by a peroxidase-antiperoxidase imiminohistochemical stain for keratin. The proposed method modifies an existing method, and expands the range of polysaccharides and mucosubstances which may be demonstrated. The proposed method is easily performed within a single working day and promises to be of value in surgical pathology as well as in studies of bronchial carcinogenesis.
AB - A method is proposed for the simultaneous staining of neutral and acidic, periodate reactive and nonperiodate reactive mucosubstances, glycogen and keratin in paraffin sections. Briefly, sections are stained by the Alcian blue (pH 2.5)-PAS method, followed by a peroxidase-antiperoxidase imiminohistochemical stain for keratin. The proposed method modifies an existing method, and expands the range of polysaccharides and mucosubstances which may be demonstrated. The proposed method is easily performed within a single working day and promises to be of value in surgical pathology as well as in studies of bronchial carcinogenesis.
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U2 - 10.3109/10520298309066789
DO - 10.3109/10520298309066789
M3 - Article
C2 - 6197775
AN - SCOPUS:84907129178
SN - 1052-0295
VL - 58
SP - 225
EP - 229
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
IS - 4
ER -