Amplification of a chromatin remodeling gene, Rsf-1/HBXAP, in ovarian carcinoma

Ie Ming Shih, Jim Jinn Chyuan Sheu, Antonio Santillan, Kentaro Nakayama, M. Jim Yen, Robert E. Bristow, Russell Vang, Giovanni Parmigiani, Robert J. Kurman, Claes G. Trope, Ben Davidson, Tian Li Wang

Research output: Contribution to journalArticlepeer-review

116 Scopus citations


A genomewide technology, digital karyotyping, was used to identify subchromosomal alterations in ovarian cancer. Amplification at 11q13.5 was found in three of seven ovarian carcinomas, and amplicon mapping delineated a 1.8-Mb core of amplification that contained 13 genes. FISH analysis demonstrated amplification of this region in 13.2% of high-grade ovarian carcinomas but not in any of low-grade carcinomas or benign ovarian tumors. Combined genetic and transcriptome analyses showed that Rsf-1 (HBXAPalpha) was the only gene that demonstrated consistent overexpression in all of the tumors harboring the 11q13.5 amplification. Patients with Rsf-1 amplification or overexpression had a significantly shorter overall survival than those without. Overexpression of Rsf-1 gene stimulated cell proliferation and transform nonneoplastic cells by conferring serum-independent and anchorage-independent growth. Furthermore, Rsf-1 gene knock-down inhibited cell growth in OVCAR3 cells, which harbor Rsf-1 amplification. Taken together, these findings indicate an important role of Rsf-1 amplification in ovarian cancer.

Original languageEnglish (US)
Pages (from-to)14004-14009
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number39
StatePublished - Sep 27 2005


  • Digital karyotyping
  • Gene amplification
  • Oncogene

ASJC Scopus subject areas

  • General


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