Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis

C. Weber; E. Gauda; E. Hecht; B. Mizaikoff; C. Kranz

doi:10.1007/978-3-642-03887-7_101

Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis

C. Weber, E. Gauda, E. Hecht, B. Mizaikoff, C. Kranz

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

An amperometric biosensor for adenosine-5′- triphosphate (ATP) was developed applying a competitive assay of two glucose converting enzymes. Hexokinase and pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase were entrapped by pH-shift induced deposition of Resydrol at a platinum microelectrode with a diameter of 25 μm. Different mediators for the PQQ-dependent glucose deydrogenase (GDH) catalyzed reaction were used applying potentials not higher than 400 mV vs. Ag/AgCl reference electrode. The obtained biosensor is selective to ATP and sensitive to it at physiological concentrations down to the nanomolar range.

Original language	English (US)
Title of host publication	World Congress on Medical Physics and Biomedical Engineering
Subtitle of host publication	Micro- and Nanosystems in Medicine, Active Implants, Biosensors
Publisher	Springer Verlag
Pages	351-354
Number of pages	4
Edition	8
ISBN (Print)	9783642038860
DOIs	https://doi.org/10.1007/978-3-642-03887-7_101
State	Published - 2009
Event	World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors - Munich, Germany Duration: Sep 7 2009 → Sep 12 2009

Publication series

Name	IFMBE Proceedings
Number	8
Volume	25
ISSN (Print)	1680-0737

Other

Other	World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors
Country/Territory	Germany
City	Munich
Period	9/7/09 → 9/12/09

Keywords

ATP
Hexokinase
Microbiosensor
PQQ-GDH
Resydrol

ASJC Scopus subject areas

Bioengineering
Biomedical Engineering

Access to Document

10.1007/978-3-642-03887-7_101

Cite this

Weber, C., Gauda, E., Hecht, E., Mizaikoff, B., & Kranz, C. (2009). Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis. In World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors (8 ed., pp. 351-354). (IFMBE Proceedings; Vol. 25, No. 8). Springer Verlag. https://doi.org/10.1007/978-3-642-03887-7_101

Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis. / Weber, C.; Gauda, E.; Hecht, E. et al.
World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors. 8. ed. Springer Verlag, 2009. p. 351-354 (IFMBE Proceedings; Vol. 25, No. 8).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Weber, C, Gauda, E, Hecht, E, Mizaikoff, B & Kranz, C 2009, Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis. in World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors. 8 edn, IFMBE Proceedings, no. 8, vol. 25, Springer Verlag, pp. 351-354, World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors, Munich, Germany, 9/7/09. https://doi.org/10.1007/978-3-642-03887-7_101

Weber C, Gauda E, Hecht E, Mizaikoff B, Kranz C. Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis. In World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors. 8 ed. Springer Verlag. 2009. p. 351-354. (IFMBE Proceedings; 8). doi: 10.1007/978-3-642-03887-7_101

Weber, C. ; Gauda, E. ; Hecht, E. et al. / Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis. World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors. 8. ed. Springer Verlag, 2009. pp. 351-354 (IFMBE Proceedings; 8).

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Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis

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Keywords

ASJC Scopus subject areas

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