TY - JOUR
T1 - Altered Th1/Th2 commitment contributes to lung senescence in CXCR3-deficient mice
AU - Huang, Junmin
AU - Li, Zongli
AU - Yao, Xiujuan
AU - Li, Yan
AU - Reng, Xiaoxia
AU - Li, Junfa
AU - Wang, Wei
AU - Gao, Jinming
AU - Wang, Chen
AU - Tankersley, Clarke G.
AU - Huang, Kewu
N1 - Funding Information:
This work was supported by the grants of National Natural Science Foundation of China ( 30871121 , 81270153 , 81170040 ) and Beijing Municipal Commission of Education ( KZ200910025007 ).
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2013/8
Y1 - 2013/8
N2 - Aging is an inevitable process associated with immune imbalance, which is characterized by a progressive functional decline in major organs, including lung. However, effects of altered Th1/Th2 commitment on lung senescence are largely unknown. To examine effects of altered Th1/Th2 balance on lung aging, we measured proportions of Th1 and Th2 cells and expression of cytokines, chemokines, collagen deposition and other relevant physiological and pathological parameters in 2- and 20-months-old (mo) CXCR3-deficient (CXCR3-/-) C57BL/6J mice compared with wild-type (WT) mice. There was a significant weight-loss observed in 20-mo CXCR3-/- mice compared with the same aged WT group. Although lung function and structure changed with age in both groups, central airway resistance (Rn), tissue elastance (H) and damping (G) were significantly lower in 20-mo CXCR3-/- mice than those of WT mice. In contrast, the whole lung volume (VL), the mean linear intercept length of alveolar (Lm), and the total lung collagen content were significantly elevated in 20-mo CXCR3-/- mice. With aging, the lungs of WT mice had typical Th1-type status (increased population of Th1 cells and concentrations of cytokine IFN-γ and CXCR3 ligands) while CXCR3-/- mice showed Th2-type polarization (decreased proportion of Th1 cells and concentrations of CXCR3 ligands but increased level of IL-4). Our data suggest that Immunosenescence is associated with lung aging, and that altered Th1/Th2 imbalance favors Th2 predominance in CXCR3-/- mice, which contributes to the process of accelerated lung aging in this model.
AB - Aging is an inevitable process associated with immune imbalance, which is characterized by a progressive functional decline in major organs, including lung. However, effects of altered Th1/Th2 commitment on lung senescence are largely unknown. To examine effects of altered Th1/Th2 balance on lung aging, we measured proportions of Th1 and Th2 cells and expression of cytokines, chemokines, collagen deposition and other relevant physiological and pathological parameters in 2- and 20-months-old (mo) CXCR3-deficient (CXCR3-/-) C57BL/6J mice compared with wild-type (WT) mice. There was a significant weight-loss observed in 20-mo CXCR3-/- mice compared with the same aged WT group. Although lung function and structure changed with age in both groups, central airway resistance (Rn), tissue elastance (H) and damping (G) were significantly lower in 20-mo CXCR3-/- mice than those of WT mice. In contrast, the whole lung volume (VL), the mean linear intercept length of alveolar (Lm), and the total lung collagen content were significantly elevated in 20-mo CXCR3-/- mice. With aging, the lungs of WT mice had typical Th1-type status (increased population of Th1 cells and concentrations of cytokine IFN-γ and CXCR3 ligands) while CXCR3-/- mice showed Th2-type polarization (decreased proportion of Th1 cells and concentrations of CXCR3 ligands but increased level of IL-4). Our data suggest that Immunosenescence is associated with lung aging, and that altered Th1/Th2 imbalance favors Th2 predominance in CXCR3-/- mice, which contributes to the process of accelerated lung aging in this model.
KW - CXCR3
KW - Immunosenescence
KW - Lung aging
KW - Lung mechanics
KW - Th1/Th2 imbalance
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U2 - 10.1016/j.exger.2013.04.001
DO - 10.1016/j.exger.2013.04.001
M3 - Article
C2 - 23583952
AN - SCOPUS:84879258128
SN - 0531-5565
VL - 48
SP - 717
EP - 7726
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 8
ER -