TY - JOUR
T1 - Alterations in protein synthesis in rat liver cells by in vitro and in vivo exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin
AU - Yasunobu, Aoki
AU - Silbergeld, Ellen K.
AU - Max, Stephen R.
AU - Fowler, Bruce A.
N1 - Funding Information:
Acknowledgements-We thank Dr J. McKinney (National Institute of Environmental Health Science) and DrsY . Takahashi and T. Miura (National Institute for En-vironmentalS tudies,J apan) for their kind gifts of TCDD and anti-ratc ytochrome-P45OIArl abbitI gd, respectively; and Dr M. M. Liuskv (Universitv of Ma&and) for his helpful suggestion.T-h is‘ work was supportedi n’ part by NIH grants( HD 41022a nd HD 16596t)o S.R.M.
PY - 1991/8/22
Y1 - 1991/8/22
N2 - Alterations in protein synthesis in rat liver cells were examined following in vitro and in vivo exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Primary cultured rat liver parenchymal cells were exposed to 1 nM TCDD for 23 and 47 hr. Synthesis of two proteins with molecular weights (Mr) of 26,000 and 39,000 (designated 26k-P and 39k-P, respectively), other than cytochrome P450, was increased markedly in the cells. These proteins did not have the same antigens as cytochromes P450IA1 and P450IA2. Synthesis of three proteins with Mrs of 24,000, 25,000 and 29,000, respectively, was decreased by TCDD. TCDD was administered to rats at a dose of 100 μg/kg body weight. The amount of five proteins (two proteins with Mr of 26,000, one of 36,000 and two of 39,000) was increased in TCDD-treated rat liver. However, the proteins increased in vivo by TCDD were distinguishable from 26k-P and 39k-P by two dimensional gel electrophoresis.
AB - Alterations in protein synthesis in rat liver cells were examined following in vitro and in vivo exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Primary cultured rat liver parenchymal cells were exposed to 1 nM TCDD for 23 and 47 hr. Synthesis of two proteins with molecular weights (Mr) of 26,000 and 39,000 (designated 26k-P and 39k-P, respectively), other than cytochrome P450, was increased markedly in the cells. These proteins did not have the same antigens as cytochromes P450IA1 and P450IA2. Synthesis of three proteins with Mrs of 24,000, 25,000 and 29,000, respectively, was decreased by TCDD. TCDD was administered to rats at a dose of 100 μg/kg body weight. The amount of five proteins (two proteins with Mr of 26,000, one of 36,000 and two of 39,000) was increased in TCDD-treated rat liver. However, the proteins increased in vivo by TCDD were distinguishable from 26k-P and 39k-P by two dimensional gel electrophoresis.
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U2 - 10.1016/0006-2952(91)90254-3
DO - 10.1016/0006-2952(91)90254-3
M3 - Article
C2 - 1888329
AN - SCOPUS:0025734464
SN - 0006-2952
VL - 42
SP - 1195
EP - 1201
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 6
ER -