Aldosterone and high-NaCl diet modulate ClC-2 chloride channel gene expression in rat kidney

Débora S. Ornellas, Danielle S. Nascimento, Daniel H. Christoph, William B. Guggino, Marcelo M. Morales

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


It is well known that Na+ reabsorption in the kidney can be regulated by aldosterone. Although Cl- is the most abundant anion present in the extra cellular fluids the involvement of aldosterone in the regulation of Cl- conductance through Cl- channels at the molecular level is unknown. In this study, the effects of aldosterone and high-Na+ diet on the expression of ClC-2, a cell volume-, pH- and voltage-sensitive Cl- channel, was examined in the rat kidney. Total RNA isolated from Wistar rats fed a high-Na+ diet for 5 days, furosemide treatment, adrenalectomy and adrenalectomy with replacement of normal plasma levels of aldosterone were compared by the use of ribonuclease protection assay (RPA), and/or a semi-quantitative RT-PCR. The high-Na+ diet reduced renal mRNA and protein ClC-2 expression. The renal expression of ClC-2 mRNA decreased in adrenalectomized rats and was restored by plasma aldosterone replacement. In addition, the semi-quantitative RT-PCR in different segments of the nephron showed that these changes were secondary to the modulation of ClC-2 mRNA expression by aldosterone in the cortical and medullary segments of thick ascending limbs of Henle's loop. These results suggest that ClC-2 may be involved with aldosterone-induced Cl- transport in the kidney.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalPflugers Archiv European Journal of Physiology
Issue number1-2
StatePublished - 2002


  • Aldosterone
  • Chloride channel
  • ClC-2
  • Kidney
  • Nephron
  • RT-PCR
  • Rat
  • Ribonuclease protection assay
  • mRNA

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)


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