Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.
ASJC Scopus subject areas
- Molecular Biology