Age-related changes in tetrahydrobiopterin and GTP-cyclohydrolase activity in the brain and adrenal gland of rats

Mir Ahamed Hossain; Joseph M. Masserano; Norman Weiner

doi:10.1016/0197-4580(95)00045-G

Age-related changes in tetrahydrobiopterin and GTP-cyclohydrolase activity in the brain and adrenal gland of rats

Mir Ahamed Hossain, Joseph M. Masserano, Norman Weiner

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In the present study, we observed that tetrahydrobiopterin (BH4) levels were decreased significantly in the striatum, substantia nigra, frontal cortex, hypothalamus, and cerebellum of 25-month-old and in the cerebellum only in case of 18-month-old rats as compared to 4-month-old rats. In contrast, BH4 levels in adrenal glands of 25-month-old rats were increased significantly. Kinetic analysis of GTP-cyclohydrolase revealed a decrease in the apparent K_m along with an increase in V_max value in the adrenal of 25-month-old rats compared to 4-month-old rats. Whereas, in cerebellum we observed that the apparent K_m of the high affinity form of the enzyme was increased and a decrease in the V_max value of the low affinity form only in case of 25-month-old rats compared to the young animals. These alterations in the BH₄ levels and its synthesizing enzyme kinetics in specific brain areas and adrenal glands of aged rats are consistent with the reported changes in the catecholamine function in central and peripheral nervous system with aging.

Original language	English (US)
Pages (from-to)	627-632
Number of pages	6
Journal	Neurobiology of aging
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/0197-4580(95)00045-G
State	Published - 1995
Externally published	Yes

Keywords

Adrenal glands
Aging
Central nervous system
GTP-cyclohydrolase
Tetrahydrobiopterin

ASJC Scopus subject areas

Neuroscience(all)
Aging
Clinical Neurology
Developmental Biology
Geriatrics and Gerontology

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10.1016/0197-4580(95)00045-G

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title = "Age-related changes in tetrahydrobiopterin and GTP-cyclohydrolase activity in the brain and adrenal gland of rats",

abstract = "In the present study, we observed that tetrahydrobiopterin (BH4) levels were decreased significantly in the striatum, substantia nigra, frontal cortex, hypothalamus, and cerebellum of 25-month-old and in the cerebellum only in case of 18-month-old rats as compared to 4-month-old rats. In contrast, BH4 levels in adrenal glands of 25-month-old rats were increased significantly. Kinetic analysis of GTP-cyclohydrolase revealed a decrease in the apparent Km along with an increase in Vmax value in the adrenal of 25-month-old rats compared to 4-month-old rats. Whereas, in cerebellum we observed that the apparent Km of the high affinity form of the enzyme was increased and a decrease in the Vmax value of the low affinity form only in case of 25-month-old rats compared to the young animals. These alterations in the BH4 levels and its synthesizing enzyme kinetics in specific brain areas and adrenal glands of aged rats are consistent with the reported changes in the catecholamine function in central and peripheral nervous system with aging.",

keywords = "Adrenal glands, Aging, Central nervous system, GTP-cyclohydrolase, Tetrahydrobiopterin",

author = "Hossain, {Mir Ahamed} and Masserano, {Joseph M.} and Norman Weiner",

year = "1995",

doi = "10.1016/0197-4580(95)00045-G",

language = "English (US)",

volume = "16",

pages = "627--632",

journal = "Neurobiology of aging",

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AU - Hossain, Mir Ahamed

AU - Masserano, Joseph M.

AU - Weiner, Norman

PY - 1995

Y1 - 1995

N2 - In the present study, we observed that tetrahydrobiopterin (BH4) levels were decreased significantly in the striatum, substantia nigra, frontal cortex, hypothalamus, and cerebellum of 25-month-old and in the cerebellum only in case of 18-month-old rats as compared to 4-month-old rats. In contrast, BH4 levels in adrenal glands of 25-month-old rats were increased significantly. Kinetic analysis of GTP-cyclohydrolase revealed a decrease in the apparent Km along with an increase in Vmax value in the adrenal of 25-month-old rats compared to 4-month-old rats. Whereas, in cerebellum we observed that the apparent Km of the high affinity form of the enzyme was increased and a decrease in the Vmax value of the low affinity form only in case of 25-month-old rats compared to the young animals. These alterations in the BH4 levels and its synthesizing enzyme kinetics in specific brain areas and adrenal glands of aged rats are consistent with the reported changes in the catecholamine function in central and peripheral nervous system with aging.

AB - In the present study, we observed that tetrahydrobiopterin (BH4) levels were decreased significantly in the striatum, substantia nigra, frontal cortex, hypothalamus, and cerebellum of 25-month-old and in the cerebellum only in case of 18-month-old rats as compared to 4-month-old rats. In contrast, BH4 levels in adrenal glands of 25-month-old rats were increased significantly. Kinetic analysis of GTP-cyclohydrolase revealed a decrease in the apparent Km along with an increase in Vmax value in the adrenal of 25-month-old rats compared to 4-month-old rats. Whereas, in cerebellum we observed that the apparent Km of the high affinity form of the enzyme was increased and a decrease in the Vmax value of the low affinity form only in case of 25-month-old rats compared to the young animals. These alterations in the BH4 levels and its synthesizing enzyme kinetics in specific brain areas and adrenal glands of aged rats are consistent with the reported changes in the catecholamine function in central and peripheral nervous system with aging.

KW - Adrenal glands

KW - Aging

KW - Central nervous system

KW - GTP-cyclohydrolase

KW - Tetrahydrobiopterin

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Age-related changes in tetrahydrobiopterin and GTP-cyclohydrolase activity in the brain and adrenal gland of rats

Abstract

Keywords

ASJC Scopus subject areas

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