Advanced Glycation End Products and Receptors in Fuchs' Dystrophy Corneas Undergoing Descemet's Stripping with Endothelial Keratoplasty

Zhiyou Wang; James T. Handa; W. Richard Green; Walter J. Stark; Robert S. Weinberg; Albert S. Jun

doi:10.1016/j.ophtha.2006.10.049

Advanced Glycation End Products and Receptors in Fuchs' Dystrophy Corneas Undergoing Descemet's Stripping with Endothelial Keratoplasty

Zhiyou Wang, James T. Handa, W. Richard Green, Walter J. Stark, Robert S. Weinberg, Albert S. Jun

School of Medicine

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Purpose: To describe the histopathologic features of Descemet's membrane (DM) obtained from Fuchs' endothelial corneal dystrophy (FECD) corneas undergoing Descemet's stripping with endothelial keratoplasty (DSEK) and to assess the presence of advanced glycation end products (AGEs) and their receptors in FECD endothelium and DM. Design: Prospective observational case series. Participants: Five eyes of 5 patients undergoing DSEK for FECD and 4 normal control eyebank corneas. Methods: Descemet's membrane and corneal endothelium from FECD patients undergoing DSEK were assessed with hematoxylin-eosin staining and immunohistochemistry for AGEs, receptor of AGEs (RAGE), and galectin 3 (AGE-R3). Main Outcome Measures: Histopathologic abnormalities and presence of AGEs, RAGE, and AGE-R3 in DSEK specimens. Results: Histopathologic assessment of DSEK specimens from FECD patients disclosed thickening and nodularity of DM and loss of endothelial cells. Immunohistochemical staining of FECD DM for AGE, RAGE, and AGE-R3 showed an abundance of AGEs in the anterior portion of DM, mild positivity for RAGE, and moderate positivity for AGE-R3. Conclusions: Tissue quality after DSEK is sufficient to allow detailed histopathologic analysis. The presence of AGEs, RAGE, and AGE-R3 in DM and corneal endothelium of FECD patients supports a link between accumulation of AGEs, oxidative stress, and corneal endothelial cell apoptosis in the pathogenesis of FECD.

Original language	English (US)
Pages (from-to)	1453-1460
Number of pages	8
Journal	Ophthalmology
Volume	114
Issue number	8
DOIs	https://doi.org/10.1016/j.ophtha.2006.10.049
State	Published - Aug 2007

ASJC Scopus subject areas

Ophthalmology

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10.1016/j.ophtha.2006.10.049

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@article{f2e8e5fcb8044d45b844f0a255fe00dc,

title = "Advanced Glycation End Products and Receptors in Fuchs' Dystrophy Corneas Undergoing Descemet's Stripping with Endothelial Keratoplasty",

abstract = "Purpose: To describe the histopathologic features of Descemet's membrane (DM) obtained from Fuchs' endothelial corneal dystrophy (FECD) corneas undergoing Descemet's stripping with endothelial keratoplasty (DSEK) and to assess the presence of advanced glycation end products (AGEs) and their receptors in FECD endothelium and DM. Design: Prospective observational case series. Participants: Five eyes of 5 patients undergoing DSEK for FECD and 4 normal control eyebank corneas. Methods: Descemet's membrane and corneal endothelium from FECD patients undergoing DSEK were assessed with hematoxylin-eosin staining and immunohistochemistry for AGEs, receptor of AGEs (RAGE), and galectin 3 (AGE-R3). Main Outcome Measures: Histopathologic abnormalities and presence of AGEs, RAGE, and AGE-R3 in DSEK specimens. Results: Histopathologic assessment of DSEK specimens from FECD patients disclosed thickening and nodularity of DM and loss of endothelial cells. Immunohistochemical staining of FECD DM for AGE, RAGE, and AGE-R3 showed an abundance of AGEs in the anterior portion of DM, mild positivity for RAGE, and moderate positivity for AGE-R3. Conclusions: Tissue quality after DSEK is sufficient to allow detailed histopathologic analysis. The presence of AGEs, RAGE, and AGE-R3 in DM and corneal endothelium of FECD patients supports a link between accumulation of AGEs, oxidative stress, and corneal endothelial cell apoptosis in the pathogenesis of FECD.",

author = "Zhiyou Wang and Handa, {James T.} and Green, {W. Richard} and Stark, {Walter J.} and Weinberg, {Robert S.} and Jun, {Albert S.}",

note = "Funding Information: Financial support: Research to Prevent Blindness, New York, New York (Career Development Award [ASJ], Clinician Scientist Award [JTH], and unrestricted grant to Wilmer Eye Institute); Medical Illness Counseling Center, Chevy Chase, Maryland (Research Grant [ASJ]); and National Institutes of Health, Bethesda, Maryland (grant nos. EY015523 [ASJ], EY14005 [JTH]). ",

year = "2007",

month = aug,

doi = "10.1016/j.ophtha.2006.10.049",

language = "English (US)",

volume = "114",

pages = "1453--1460",

journal = "Ophthalmology",

issn = "0161-6420",

publisher = "Elsevier Inc.",

number = "8",

}

TY - JOUR

T1 - Advanced Glycation End Products and Receptors in Fuchs' Dystrophy Corneas Undergoing Descemet's Stripping with Endothelial Keratoplasty

AU - Wang, Zhiyou

AU - Handa, James T.

AU - Green, W. Richard

AU - Stark, Walter J.

AU - Weinberg, Robert S.

AU - Jun, Albert S.

N1 - Funding Information: Financial support: Research to Prevent Blindness, New York, New York (Career Development Award [ASJ], Clinician Scientist Award [JTH], and unrestricted grant to Wilmer Eye Institute); Medical Illness Counseling Center, Chevy Chase, Maryland (Research Grant [ASJ]); and National Institutes of Health, Bethesda, Maryland (grant nos. EY015523 [ASJ], EY14005 [JTH]).

PY - 2007/8

Y1 - 2007/8

N2 - Purpose: To describe the histopathologic features of Descemet's membrane (DM) obtained from Fuchs' endothelial corneal dystrophy (FECD) corneas undergoing Descemet's stripping with endothelial keratoplasty (DSEK) and to assess the presence of advanced glycation end products (AGEs) and their receptors in FECD endothelium and DM. Design: Prospective observational case series. Participants: Five eyes of 5 patients undergoing DSEK for FECD and 4 normal control eyebank corneas. Methods: Descemet's membrane and corneal endothelium from FECD patients undergoing DSEK were assessed with hematoxylin-eosin staining and immunohistochemistry for AGEs, receptor of AGEs (RAGE), and galectin 3 (AGE-R3). Main Outcome Measures: Histopathologic abnormalities and presence of AGEs, RAGE, and AGE-R3 in DSEK specimens. Results: Histopathologic assessment of DSEK specimens from FECD patients disclosed thickening and nodularity of DM and loss of endothelial cells. Immunohistochemical staining of FECD DM for AGE, RAGE, and AGE-R3 showed an abundance of AGEs in the anterior portion of DM, mild positivity for RAGE, and moderate positivity for AGE-R3. Conclusions: Tissue quality after DSEK is sufficient to allow detailed histopathologic analysis. The presence of AGEs, RAGE, and AGE-R3 in DM and corneal endothelium of FECD patients supports a link between accumulation of AGEs, oxidative stress, and corneal endothelial cell apoptosis in the pathogenesis of FECD.

AB - Purpose: To describe the histopathologic features of Descemet's membrane (DM) obtained from Fuchs' endothelial corneal dystrophy (FECD) corneas undergoing Descemet's stripping with endothelial keratoplasty (DSEK) and to assess the presence of advanced glycation end products (AGEs) and their receptors in FECD endothelium and DM. Design: Prospective observational case series. Participants: Five eyes of 5 patients undergoing DSEK for FECD and 4 normal control eyebank corneas. Methods: Descemet's membrane and corneal endothelium from FECD patients undergoing DSEK were assessed with hematoxylin-eosin staining and immunohistochemistry for AGEs, receptor of AGEs (RAGE), and galectin 3 (AGE-R3). Main Outcome Measures: Histopathologic abnormalities and presence of AGEs, RAGE, and AGE-R3 in DSEK specimens. Results: Histopathologic assessment of DSEK specimens from FECD patients disclosed thickening and nodularity of DM and loss of endothelial cells. Immunohistochemical staining of FECD DM for AGE, RAGE, and AGE-R3 showed an abundance of AGEs in the anterior portion of DM, mild positivity for RAGE, and moderate positivity for AGE-R3. Conclusions: Tissue quality after DSEK is sufficient to allow detailed histopathologic analysis. The presence of AGEs, RAGE, and AGE-R3 in DM and corneal endothelium of FECD patients supports a link between accumulation of AGEs, oxidative stress, and corneal endothelial cell apoptosis in the pathogenesis of FECD.

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SN - 0161-6420

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JO - Ophthalmology

JF - Ophthalmology

IS - 8

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Advanced Glycation End Products and Receptors in Fuchs' Dystrophy Corneas Undergoing Descemet's Stripping with Endothelial Keratoplasty

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