TY - JOUR
T1 - Adult-onset, chronic, cyclic thrombocytopenia in a Rhesus macaque (Macaca mulatta) after dengue virus vaccination and viral challenge
AU - Frydman, Galit H.
AU - Metcalf Pate, Kelly A.
AU - Marini, Robert P.
AU - de Laforcade, Armelle M.
AU - Bosch, Irene
AU - Bakthavatchalu, Vasudevan
AU - Muthupalani, Sureshkumar
AU - Swennes, Alton G.
AU - Lyons, Claire E.
AU - Tompkins, Ronald G.
AU - Fox, James G.
N1 - Publisher Copyright:
© 2017 American Society for Veterinary Clinical Pathology
PY - 2017/6
Y1 - 2017/6
N2 - An 8-year-old, male Rhesus macaque (Macaca mulatta), previously used for dengue virus (DENV) vaccine research with viral challenge, was presented with adult-onset, chronic, cyclic thrombocytopenia. Platelet number, morphology, and function were evaluated by automated hematology, peripheral blood smears, electron microscopy, flow cytometry, and impedance aggregometry. Bone marrow was evaluated by cytology. Both serum anti-dengue nonstructural protein 1 (NS1) antibodies and anti-platelet antibodies were detected by ELISA. Platelet characterization showed a lack of aggregation to all agonists (ADP, ASP, and collagen), increased activation with increased expression of surface marker (HLA-ABC), and an absence of surface receptor GPIX during clinical episodes of petechiae and ecchymoses, even in the presence of normal platelet counts. Bone marrow aspirates identified potential mild megakaryocytic hypoplasia. All platelet functions and morphologic attributes were within normal limits during clinically normal phases. Presence of anti-dengue NS1 serum antibodies confirmed a positive DENV titer 8 years postvaccination. Based on the history and clinical findings, a primary differential diagnosis for this chronic, cyclic platelet pathology was autoimmune platelet destruction with potential bone marrow involvement.
AB - An 8-year-old, male Rhesus macaque (Macaca mulatta), previously used for dengue virus (DENV) vaccine research with viral challenge, was presented with adult-onset, chronic, cyclic thrombocytopenia. Platelet number, morphology, and function were evaluated by automated hematology, peripheral blood smears, electron microscopy, flow cytometry, and impedance aggregometry. Bone marrow was evaluated by cytology. Both serum anti-dengue nonstructural protein 1 (NS1) antibodies and anti-platelet antibodies were detected by ELISA. Platelet characterization showed a lack of aggregation to all agonists (ADP, ASP, and collagen), increased activation with increased expression of surface marker (HLA-ABC), and an absence of surface receptor GPIX during clinical episodes of petechiae and ecchymoses, even in the presence of normal platelet counts. Bone marrow aspirates identified potential mild megakaryocytic hypoplasia. All platelet functions and morphologic attributes were within normal limits during clinically normal phases. Presence of anti-dengue NS1 serum antibodies confirmed a positive DENV titer 8 years postvaccination. Based on the history and clinical findings, a primary differential diagnosis for this chronic, cyclic platelet pathology was autoimmune platelet destruction with potential bone marrow involvement.
KW - Autoimmune
KW - ITP
KW - nonhuman primate
KW - platelets
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U2 - 10.1111/vcp.12497
DO - 10.1111/vcp.12497
M3 - Article
C2 - 28518476
AN - SCOPUS:85019913378
SN - 0275-6382
VL - 46
SP - 238
EP - 247
JO - Bulletin of the American Society of Veterinary Clinical Pathologists
JF - Bulletin of the American Society of Veterinary Clinical Pathologists
IS - 2
ER -