Adsorption, retention and biologic activity of proteins adsorbed on gas discharge treated surfaces

A. S. Hoffman; D. Kiaei; A. Safranj; J. P. Chen; A. Johnstona; T. A. Horbett; F. Zavala; A. M. Deelder; J. B. Castelino; V. Markovic

doi:10.1016/0267-6605(92)90033-P

Adsorption, retention and biologic activity of proteins adsorbed on gas discharge treated surfaces

A. S. Hoffman, D. Kiaei, A. Safranj, J. P. Chen, A. Johnstona, T. A. Horbett, F. Zavala, A. M. Deelder, J. B. Castelino, V. Markovic

Research output: Contribution to journal › Article › peer-review

Abstract

We have observed that proteins adsorb very tenaciously to polymer surfaces that have been treated with certain gases in a gas discharge. These gases include tetrachoroethylene (TFE) and tetrachloroethylene (TCE). We have studied adsorption of plasma proteins, antibodies, antigens and enzymes. In this article we present results on the adsorption, retention and biologic activity of (a) albumin as a passivating surface, (b) malaria and schistosomiasis antibodies in an immunoassay and (c) horseradish peroxidase (HRP) as a model enzyme biosensor. In some cases, the protein adsorbed to the treated surface retains greater activity and/or greater stability when compared to the untreated control surface. We also discuss the possible mechanism(s) of these interesting and unusual effects.

Original language	English (US)
Pages (from-to)	93-98
Number of pages	6
Journal	Clinical Materials
Volume	11
Issue number	1-4
DOIs	https://doi.org/10.1016/0267-6605(92)90033-P
State	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

Biophysics

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10.1016/0267-6605(92)90033-P

Cite this

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title = "Adsorption, retention and biologic activity of proteins adsorbed on gas discharge treated surfaces",

abstract = "We have observed that proteins adsorb very tenaciously to polymer surfaces that have been treated with certain gases in a gas discharge. These gases include tetrachoroethylene (TFE) and tetrachloroethylene (TCE). We have studied adsorption of plasma proteins, antibodies, antigens and enzymes. In this article we present results on the adsorption, retention and biologic activity of (a) albumin as a passivating surface, (b) malaria and schistosomiasis antibodies in an immunoassay and (c) horseradish peroxidase (HRP) as a model enzyme biosensor. In some cases, the protein adsorbed to the treated surface retains greater activity and/or greater stability when compared to the untreated control surface. We also discuss the possible mechanism(s) of these interesting and unusual effects.",

author = "Hoffman, {A. S.} and D. Kiaei and A. Safranj and Chen, {J. P.} and A. Johnstona and Horbett, {T. A.} and F. Zavala and Deelder, {A. M.} and Castelino, {J. B.} and V. Markovic",

year = "1992",

doi = "10.1016/0267-6605(92)90033-P",

language = "English (US)",

volume = "11",

pages = "93--98",

journal = "Clinical Materials",

issn = "0267-6605",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Adsorption, retention and biologic activity of proteins adsorbed on gas discharge treated surfaces

AU - Hoffman, A. S.

AU - Kiaei, D.

AU - Safranj, A.

AU - Chen, J. P.

AU - Johnstona, A.

AU - Horbett, T. A.

AU - Zavala, F.

AU - Deelder, A. M.

AU - Castelino, J. B.

AU - Markovic, V.

PY - 1992

Y1 - 1992

N2 - We have observed that proteins adsorb very tenaciously to polymer surfaces that have been treated with certain gases in a gas discharge. These gases include tetrachoroethylene (TFE) and tetrachloroethylene (TCE). We have studied adsorption of plasma proteins, antibodies, antigens and enzymes. In this article we present results on the adsorption, retention and biologic activity of (a) albumin as a passivating surface, (b) malaria and schistosomiasis antibodies in an immunoassay and (c) horseradish peroxidase (HRP) as a model enzyme biosensor. In some cases, the protein adsorbed to the treated surface retains greater activity and/or greater stability when compared to the untreated control surface. We also discuss the possible mechanism(s) of these interesting and unusual effects.

AB - We have observed that proteins adsorb very tenaciously to polymer surfaces that have been treated with certain gases in a gas discharge. These gases include tetrachoroethylene (TFE) and tetrachloroethylene (TCE). We have studied adsorption of plasma proteins, antibodies, antigens and enzymes. In this article we present results on the adsorption, retention and biologic activity of (a) albumin as a passivating surface, (b) malaria and schistosomiasis antibodies in an immunoassay and (c) horseradish peroxidase (HRP) as a model enzyme biosensor. In some cases, the protein adsorbed to the treated surface retains greater activity and/or greater stability when compared to the untreated control surface. We also discuss the possible mechanism(s) of these interesting and unusual effects.

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DO - 10.1016/0267-6605(92)90033-P

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VL - 11

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EP - 98

JO - Clinical Materials

JF - Clinical Materials

IS - 1-4

ER -

Adsorption, retention and biologic activity of proteins adsorbed on gas discharge treated surfaces

Abstract

ASJC Scopus subject areas

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