Adoptive cell transfer of macrophages following peripheral nerve injury in mice

Atul Rawat, Mithilesh Kumar Jha, Brett Michael Morrison

Research output: Contribution to journalArticlepeer-review


Macrophages are key innate immune cells involved in multiple biological processes, including peripheral nerve regeneration. Here, we describe a protocol for the adoptive cell transfer of bone-marrow-derived macrophages (BMDMs) following sciatic nerve crush injury (SNCI). This procedure involves isolating BMDMs from a donor mouse, potentially manipulating them ex vivo, and reintroducing them into an animal following SNCI. Preclinical studies show that BMDMs can infiltrate injured nerves and impact functional recovery, potentially providing a novel therapy for nerve injuries. For complete details on the use and execution of this protocol, please refer to Jha et al.1

Original languageEnglish (US)
Article number102624
JournalSTAR Protocols
Issue number4
StatePublished - Dec 15 2023


  • Cell Culture
  • Immunology
  • Model Organisms

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience


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