@inbook{e79f7ae7b655419fbbb934959a3da18d,
title = "Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells",
abstract = "Multipotent mesenchymal stem cells (MSCs) are an attractive candidate for regeneration of damaged cells, tissues, and organs. Due to limited availabilities, MSC populations must be rapidly expanded to satisfy clinical needs. However, senescence attributed to extensive in vitro expansion compromises the regenerative and therapeutic potential of MSCs. In this chapter, we describe a step-by-step protocol that aims to induce adipogenic and osteogenic differentiation of in vitro aged human MSCs and highlight noteworthy issues that may arise during the process.",
keywords = "Adipogenesis, Differentiation, Expansion, Flow cytometry, Histology, Mesenchymal stem cell, Osteogenesis, Senescence",
author = "Ogando, \{Courtney R.\} and Barabino, \{Gilda A.\} and Yang, \{Yueh Hsun Kevin\}",
note = "Funding Information: This work was supported by the City College of New York Research Award (to GAB) and partially by the Professional Staff Congress ? City University of New York Research Award (Grant number 61584-00 49 to YHKY). Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2018. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2019",
doi = "10.1007/7651\_2018\_197",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "107--117",
booktitle = "Methods in Molecular Biology",
}