@article{aa8f74c2bd5a466b90f0f2d8446b8d97,
title = "Adenoviral infection of thyroid cells: A rationale for gene therapy for metastatic thyroid carcinoma",
abstract = "Background. Patients with thyroid carcinoma experience excellent long- term survival; however, up to 16% will die of their disease. We have transformed a rat thyroid follicular cell line (FRTL-5) with a gene (TGCT) that mimics a known mutation associated with thyroid neoplasms. These cells form subcutaneous tumors that metastasize to lung in nude mice. Methods. In anticipation of developing gene therapy against this thyroid carcinoma model, we (1) tested whether adenovirus containing the β-galactosidase gene could infect FRTL-5 cells and neonatal rat thyroid and (2) evaluated the ability to kill FRTL-5 cells by transfecting them with a transgene in which the thyroglobulin promoter (TG) directed the expression of herpes simplex virus type I thymidine kinase (HSV1TK) and treating TG-HSV1TK-transfected cells with 5 μg/ml ganciclovir. Results. Nearly 100% of the TG-HSV1TK but only 5% of control cells were killed by addition of ganciclovir. Histochemical staining for β-galactosidase activity demonstrated infection of FRTL-5 cells and neonatal rat thyroid tissue by adenovirus β-galactosidase. Conclusions. These data demonstrate the feasibility of using adenovirus as vector to infect thyroid cells in vivo and provide a rationale for development of gene therapy for treatment of thyroid cancer.",
author = "Zeiger, {M. A.} and Y. Takiyama and Bishop, {J. O.} and Ellison, {A. R.} and M. Saji and Levine, {M. A.} and Doherty, {G. M.} and P. Goretzki",
note = "Funding Information: ALTHOUGH WELL-DIFFERENTIATEDt hyroid carcinoma in general carries an excellent long-term prognosis, between 8% and 16% of patients with these cancers will experience recurrence of their disease and eventually die of disseminated thyroid carcinoma. 14 With the exception of radioiodine therapy and possibly external beam radiation, no efficacious therapies are available to these patients. Relevant to this, we have used molecular genetic techniques to create a metastatic thyroid carcinoma model (TGCT) in athymic nude mice (unpublished data) by mimicking a known mutation associated with well-differentiated thyroid tumors) TGCT cells are rat thyroid follicular cells (FRTL-5) that have been transfected with a minigene in which the cholera toxin Partially supported by the T.R.A.C. grant, Knoll Pharmaceutical Company. Presented at the Seventeenth Annual Meeting of the American Association of Endocrine Smgeons, Napa, Calif.,A pril 21-23, 1996. Reprint requests: MarthaA. Zeiger, MD, FACS,D epartment of Surgery, Divisiono f Surgical Oncology and Endocrine Surgeiy,Johns Hopkins University, 600 N. Wolfe St., Harvey 608, Baltimore, MD 21287-8611. Copyright 9 1996 hy Mosby-YearB ook, Inc. 0039-6060/96/$5.00 +0 11/6/75773 A1 subunit is under control of the thyroglobulin gene promoter (TG).",
year = "1996",
doi = "10.1016/S0039-6060(96)80034-1",
language = "English (US)",
volume = "120",
pages = "921--925",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "6",
}