AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

Yumi Jang; Fred Bunz

doi:10.1016/j.xpro.2022.101123

AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

Yumi Jang, Fred Bunz

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The AdenoBuilder platform enables the in vitro assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and can be applied to other parts of the HAd5 genome. For complete details on the use and execution of this protocol, please refer to Miciak et al. (2018).

Original language	English (US)
Article number	101123
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101123
State	Published - Mar 18 2022

Keywords

Biotechnology and bioengineering
Microbiology
Molecular Biology

ASJC Scopus subject areas

General Medicine
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience

Access to Document

10.1016/j.xpro.2022.101123

Cite this

@article{4e494e2372a94252a1108739f6a499fd,

title = "AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses",

abstract = "The AdenoBuilder platform enables the in vitro assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and can be applied to other parts of the HAd5 genome. For complete details on the use and execution of this protocol, please refer to Miciak et al. (2018).",

keywords = "Biotechnology and bioengineering, Microbiology, Molecular Biology",

author = "Yumi Jang and Fred Bunz",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = mar,

day = "18",

doi = "10.1016/j.xpro.2022.101123",

language = "English (US)",

volume = "3",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - AdenoBuilder

T2 - A platform for the modular assembly of recombinant adenoviruses

AU - Jang, Yumi

AU - Bunz, Fred

PY - 2022/3/18

Y1 - 2022/3/18

N2 - The AdenoBuilder platform enables the in vitro assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and can be applied to other parts of the HAd5 genome. For complete details on the use and execution of this protocol, please refer to Miciak et al. (2018).

AB - The AdenoBuilder platform enables the in vitro assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and can be applied to other parts of the HAd5 genome. For complete details on the use and execution of this protocol, please refer to Miciak et al. (2018).

KW - Biotechnology and bioengineering

KW - Microbiology

KW - Molecular Biology

UR - http://www.scopus.com/inward/record.url?scp=85123070667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85123070667&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101123

DO - 10.1016/j.xpro.2022.101123

M3 - Article

C2 - 35098167

AN - SCOPUS:85123070667

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 101123

ER -

AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this