Abstract
Hormonal induction of 3T3-L1 preadipocytes triggers a cascade of events that initiate differentiation into adipocytes. CCAAT/enhancer-binding proteins β and δ (C/EBPβ/δ) are expressed early in the differentiation program, but are not immediately active. After a long lag, C/EBPβ/δ become competent to bind to the C/EBP regulatory element in the C/EBPα gene promoter, C/EBPα being a transcriptional activator of numerous adipocyte genes. As C/EBPβ/δ acquire binding activity, they become localized to centromeres as preadipocytes synchronously enter S phase at the onset of mitotic clonal expansion. Localization to centromeres occurs through C/EBP consensus-binding sites in centromeric satellite DNA. C/EBPα, which is antimitotic, becomes centromere-associated much later in the differentiation program as mitotic clonal expansion ceases and the cells become terminally differentiated.
Original language | English (US) |
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Pages (from-to) | 2231-2241 |
Number of pages | 11 |
Journal | Genes & development |
Volume | 13 |
Issue number | 17 |
DOIs | |
State | Published - Sep 1 1999 |
Keywords
- 3T3-L1 preadipocyte
- C/EBP
- Cell cycle
- Centromere
- Satellite DNA
ASJC Scopus subject areas
- Genetics
- Developmental Biology