Accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride gases

Frederick A. Bettelheim; J. Samuel Zigler; Venkat N. Reddy

doi:10.1016/S0014-4835(87)80150-1

Accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride gases

Frederick A. Bettelheim, J. Samuel Zigler, Venkat N. Reddy

School of Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Low-molecular-weight crystallin was isolated from bovine lenses. Its purity was established by high-pressure liquid chromatography (HPLC) and sodium dodecyl sulfate (SDS)-gel electrophoresis. The amino-acid composition, in general, and the total available acidic and basic sites, in particular, were obtained by amino-acid analysis. The accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride vapor was obtained by high vacuum vapor sorption. The sorption and desorption isotherms were analysed regarding the gas uptake at different vapor pressures. The amount of ammonia and hydrogen chloride irreversibly sorbed and retained as well as the hysteresis imply high accessibility of crystallins to the polar gases. The results indicate that molecular self aggregation of low-molecular-weight crystallins in solid state occurs mostly through hydrophobic domains.

Original language	English (US)
Pages (from-to)	271-279
Number of pages	9
Journal	Experimental eye research
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/S0014-4835(87)80150-1
State	Published - Aug 1987

Keywords

accessibility
crystallin
molecular self aggregation
vapor sorption

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/S0014-4835(87)80150-1

Cite this

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title = "Accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride gases",

abstract = "Low-molecular-weight crystallin was isolated from bovine lenses. Its purity was established by high-pressure liquid chromatography (HPLC) and sodium dodecyl sulfate (SDS)-gel electrophoresis. The amino-acid composition, in general, and the total available acidic and basic sites, in particular, were obtained by amino-acid analysis. The accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride vapor was obtained by high vacuum vapor sorption. The sorption and desorption isotherms were analysed regarding the gas uptake at different vapor pressures. The amount of ammonia and hydrogen chloride irreversibly sorbed and retained as well as the hysteresis imply high accessibility of crystallins to the polar gases. The results indicate that molecular self aggregation of low-molecular-weight crystallins in solid state occurs mostly through hydrophobic domains.",

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author = "Bettelheim, {Frederick A.} and Zigler, {J. Samuel} and Reddy, {Venkat N.}",

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TY - JOUR

T1 - Accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride gases

AU - Bettelheim, Frederick A.

AU - Zigler, J. Samuel

AU - Reddy, Venkat N.

PY - 1987/8

Y1 - 1987/8

N2 - Low-molecular-weight crystallin was isolated from bovine lenses. Its purity was established by high-pressure liquid chromatography (HPLC) and sodium dodecyl sulfate (SDS)-gel electrophoresis. The amino-acid composition, in general, and the total available acidic and basic sites, in particular, were obtained by amino-acid analysis. The accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride vapor was obtained by high vacuum vapor sorption. The sorption and desorption isotherms were analysed regarding the gas uptake at different vapor pressures. The amount of ammonia and hydrogen chloride irreversibly sorbed and retained as well as the hysteresis imply high accessibility of crystallins to the polar gases. The results indicate that molecular self aggregation of low-molecular-weight crystallins in solid state occurs mostly through hydrophobic domains.

AB - Low-molecular-weight crystallin was isolated from bovine lenses. Its purity was established by high-pressure liquid chromatography (HPLC) and sodium dodecyl sulfate (SDS)-gel electrophoresis. The amino-acid composition, in general, and the total available acidic and basic sites, in particular, were obtained by amino-acid analysis. The accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride vapor was obtained by high vacuum vapor sorption. The sorption and desorption isotherms were analysed regarding the gas uptake at different vapor pressures. The amount of ammonia and hydrogen chloride irreversibly sorbed and retained as well as the hysteresis imply high accessibility of crystallins to the polar gases. The results indicate that molecular self aggregation of low-molecular-weight crystallins in solid state occurs mostly through hydrophobic domains.

KW - accessibility

KW - crystallin

KW - molecular self aggregation

KW - vapor sorption

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Accessibility of low-molecular-weight crystallins to ammonia and hydrogen chloride gases

Abstract

Keywords

ASJC Scopus subject areas

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