Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions

T. Tsukamoto, T. Hikida

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The interactions between pyrene and bovine serum albumin (BSA) and pyrene and caffeine were studied using absorption spectroscopy in aqueous solutions. In the pyrene-BSA system, an equilibrium between free and adsorbed pyrene was observed in the solution. The absorption spectrum of pyrene adsorbed by BSA was obtained. The equilibrium constant was determined to be 1.1 × 106 dm3 mol-1. In the presence of caffeine, the absorption spectrum was a superposition of three species; free pyrene and 1:1 and 1:2 pyrene-caffeine complexes. The equilibrium constants were determined to be 820 and 260 dm3 mol-1 for the formation reactions of 1:1 and 1:2 pyrene-caffeine complexes respectively.

Original languageEnglish (US)
Pages (from-to)271-273
Number of pages3
JournalJournal of Photochemistry and Photobiology A: Chemistry
Issue number3
StatePublished - May 10 1996
Externally publishedYes


  • Absorption spectra
  • Bovine serum albumin
  • Caffeine
  • Complex
  • Pyrene

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering
  • General Physics and Astronomy


Dive into the research topics of 'Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions'. Together they form a unique fingerprint.

Cite this