Aberrant wound-healing response in mitomycin C-treated leaking blebs: A histopathologic study

Victor M. Elner, Paula Anne Newman-Casey, A. Jayaprakash Patil, Andrew Flint, Jyotirmay Biswas, Sayoko E. Moroi, Vaijayanthi Pushparaj, Deepak P. Edward

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Objective: To characterize histopathologic features of leaking mitomycin C-treated blebs and aberrant wound healing that may lead to persistent conjunctival thinning and leakage. Methods: Forty mitomycin C-treated filtering blebs excised for persistent leaks from 40 patients were examined histopathologically using hematoxylin-eosin, periodic acid-Schiff, Masson trichrome, and Alcian blue histochemical stains. Results: Ninety percent of the leaking blebs contained epithelial-stromal domes with areas of acellular stroma covered by attenuated epithelium. Seventy-five percent of the blebs demonstrated varying degrees of fibrovascular repair growing from the bleb margin, either beneath or interdigitating with the acellular zone. A novel observation in 65% of specimens was Alcian blue-positive myxoid stroma at the interface between the fibrovascular proliferation and the epithelial-stromal dome. The association between the presence of fibrovascular proliferation and Alcian blue-staining myxoid stroma was significant by Fisher exact test (P = .002). Conclusions: A desirable filtration bleb requires a sufficient reparative fibrovascular response to maintain an epithelial-stromal barrier to prevent leakage. Fibroblasts must lay down a continuous collagen-rich fibrous layer, rather than merely myxoid stroma, beneath the conjunctival epithelium to promote bleb stability. Surgical techniques and postsurgical care should aim to attain this desired outcome.

Original languageEnglish (US)
Pages (from-to)1036-1042
Number of pages7
JournalArchives of Ophthalmology
Issue number8
StatePublished - Aug 2009
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology


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