Aberrant DNA methylation of the transcription factor C/EBPα in acute myelogenous leukemia

Edgar Jost; Nicole do Ó; Stefan Wilop; James G. Herman; Rainhardt Osieka; Oliver Galm

doi:10.1016/j.leukres.2008.07.027

Aberrant DNA methylation of the transcription factor C/EBPα in acute myelogenous leukemia

Edgar Jost, Nicole do Ó, Stefan Wilop, James G. Herman, Rainhardt Osieka, Oliver Galm

School of Medicine

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We investigated the methylation status of the CCAAT/enhancer binding protein alpha (C/EBPα) promoter region near the transcription start site in acute myelogenous leukemia (AML). In hematopoietic tumor cell lines, CpG island hypermethylation of the proximal C/EBPα promoter region was associated with transcriptional silencing, and treatment with the demethylating agent 5-aza-2′-deoxycytidine resulted in C/EBPα reexpression and promoter demethylation. Aberrant methylation of the C/EBPα promoter region occurred in 10/80 diagnostic AML samples, and there was an inverse correlation between aberrant methylation of C/EBPα and the negative cell cycle regulator p15. Our results provide further evidence for epigenetic dysregulation of C/EBPα in AML.

Original language	English (US)
Pages (from-to)	443-449
Number of pages	7
Journal	Leukemia Research
Volume	33
Issue number	3
DOIs	https://doi.org/10.1016/j.leukres.2008.07.027
State	Published - Mar 2009

Keywords

Acute myelogenous leukemia
C/EBP
Cell cycle
DNA methylation
Epigenetics
Transcriptional silencing

ASJC Scopus subject areas

Cancer Research
Hematology
Oncology

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10.1016/j.leukres.2008.07.027

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title = "Aberrant DNA methylation of the transcription factor C/EBPα in acute myelogenous leukemia",

abstract = "We investigated the methylation status of the CCAAT/enhancer binding protein alpha (C/EBPα) promoter region near the transcription start site in acute myelogenous leukemia (AML). In hematopoietic tumor cell lines, CpG island hypermethylation of the proximal C/EBPα promoter region was associated with transcriptional silencing, and treatment with the demethylating agent 5-aza-2′-deoxycytidine resulted in C/EBPα reexpression and promoter demethylation. Aberrant methylation of the C/EBPα promoter region occurred in 10/80 diagnostic AML samples, and there was an inverse correlation between aberrant methylation of C/EBPα and the negative cell cycle regulator p15. Our results provide further evidence for epigenetic dysregulation of C/EBPα in AML.",

keywords = "Acute myelogenous leukemia, C/EBP, Cell cycle, DNA methylation, Epigenetics, Transcriptional silencing",

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AU - Jost, Edgar

AU - Ó, Nicole do

AU - Wilop, Stefan

AU - Herman, James G.

AU - Osieka, Rainhardt

AU - Galm, Oliver

PY - 2009/3

Y1 - 2009/3

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AB - We investigated the methylation status of the CCAAT/enhancer binding protein alpha (C/EBPα) promoter region near the transcription start site in acute myelogenous leukemia (AML). In hematopoietic tumor cell lines, CpG island hypermethylation of the proximal C/EBPα promoter region was associated with transcriptional silencing, and treatment with the demethylating agent 5-aza-2′-deoxycytidine resulted in C/EBPα reexpression and promoter demethylation. Aberrant methylation of the C/EBPα promoter region occurred in 10/80 diagnostic AML samples, and there was an inverse correlation between aberrant methylation of C/EBPα and the negative cell cycle regulator p15. Our results provide further evidence for epigenetic dysregulation of C/EBPα in AML.

KW - Acute myelogenous leukemia

KW - C/EBP

KW - Cell cycle

KW - DNA methylation

KW - Epigenetics

KW - Transcriptional silencing

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Aberrant DNA methylation of the transcription factor C/EBPα in acute myelogenous leukemia

Abstract

Keywords

ASJC Scopus subject areas

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