A yeast protein, homologous to the proteolipid of the chromaffin granule proton-ATPase, is important for cell growth

Michelle Apperson; Robert E. Jensen; Kitaru Suda; Christine Witte; Michael P. Yaffe

doi:10.1016/0006-291X(90)92359-8

A yeast protein, homologous to the proteolipid of the chromaffin granule proton-ATPase, is important for cell growth

Michelle Apperson
, Robert E. Jensen
, Kitaru Suda
, Christine Witte
, Michael P. Yaffe

School of Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We have characterized a gene, PPA1, adjacent to the yeast MAS2 gene. DNA sequence analysis of PPA1 predicts a hydrophobic protein of 23 kDa. This protein is homologous to the proteolipid of the bovine chromaffin granule proton ATPase and to the proteolipid of the yeast vacuolar proton ATPase. Gene disruption experiments indicate that the PPA1 protein is essential for viability in three unrelated yeast strains and important for optimal growth in a fourth strain.

Original language	English (US)
Pages (from-to)	574-579
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	168
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)92359-8
State	Published - Apr 30 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "A yeast protein, homologous to the proteolipid of the chromaffin granule proton-ATPase, is important for cell growth",

abstract = "We have characterized a gene, PPA1, adjacent to the yeast MAS2 gene. DNA sequence analysis of PPA1 predicts a hydrophobic protein of 23 kDa. This protein is homologous to the proteolipid of the bovine chromaffin granule proton ATPase and to the proteolipid of the yeast vacuolar proton ATPase. Gene disruption experiments indicate that the PPA1 protein is essential for viability in three unrelated yeast strains and important for optimal growth in a fourth strain.",

author = "Michelle Apperson and Jensen, \{Robert E.\} and Kitaru Suda and Christine Witte and Yaffe, \{Michael P.\}",

note = "Funding Information: Acknowledgments: We thank Jeff analysis. This work was supported from the Chicago Community Trust.",

year = "1990",

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T1 - A yeast protein, homologous to the proteolipid of the chromaffin granule proton-ATPase, is important for cell growth

AU - Apperson, Michelle

AU - Jensen, Robert E.

AU - Suda, Kitaru

AU - Witte, Christine

AU - Yaffe, Michael P.

N1 - Funding Information: Acknowledgments: We thank Jeff analysis. This work was supported from the Chicago Community Trust.

PY - 1990/4/30

Y1 - 1990/4/30

N2 - We have characterized a gene, PPA1, adjacent to the yeast MAS2 gene. DNA sequence analysis of PPA1 predicts a hydrophobic protein of 23 kDa. This protein is homologous to the proteolipid of the bovine chromaffin granule proton ATPase and to the proteolipid of the yeast vacuolar proton ATPase. Gene disruption experiments indicate that the PPA1 protein is essential for viability in three unrelated yeast strains and important for optimal growth in a fourth strain.

AB - We have characterized a gene, PPA1, adjacent to the yeast MAS2 gene. DNA sequence analysis of PPA1 predicts a hydrophobic protein of 23 kDa. This protein is homologous to the proteolipid of the bovine chromaffin granule proton ATPase and to the proteolipid of the yeast vacuolar proton ATPase. Gene disruption experiments indicate that the PPA1 protein is essential for viability in three unrelated yeast strains and important for optimal growth in a fourth strain.

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UR - https://www.scopus.com/pages/publications/0025290460#tab=citedBy

U2 - 10.1016/0006-291X(90)92359-8

DO - 10.1016/0006-291X(90)92359-8

M3 - Article

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AN - SCOPUS:0025290460

SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

A yeast protein, homologous to the proteolipid of the chromaffin granule proton-ATPase, is important for cell growth

Abstract

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