@inproceedings{d2fc9ab3eb0341babd499ce3620904fb,
title = "A vacuum-driven microfluidic array for multi-step sample digitalization",
abstract = "There remains an unmet need for simple microfluidic platforms that can digitize and perform multi-step and multi-reagent biochemical assays in parallel for high-throughput detection and analysis of single molecules and single cells. In response, we report herein a vacuum-driven microfluidic chip that is capable of multi-step sample digitalization based on a tree-like multi-level bifurcation structure and gas permeability of PDMS. Multi-step loading capability and loading uniformity in each step were characterized, showing strong performance of our device. With further development, our platform can provide a promising tool for achieving high-throughput digital multi-step, multi-reagent biochemical assays.",
keywords = "Digitalization, High-throughput, Multi-step assay, Vacuum-driven",
author = "Jiumei Hu and Liben Chen and Hui Li and Kuangwen Hsieh and Pengfei Zhang and Wang, \{Tza Huei\}",
note = "Publisher Copyright: {\textcopyright} 2020 CBMS-0001; 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",
year = "2020",
language = "English (US)",
series = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "274--275",
booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}