@inproceedings{d81b845dbaf54ae88dd0313757fad74e,
title = "A Vacuum-Driven Microfluidic Array for Multi-Step Sample Digitalization",
abstract = "We present herein a facile vacuum-driven microfluidic device that is capable of multi-step sample digitalization based on the gas permeability of PDMS. The device features 1) a suction layer that is connected to an external vacuum to generate a continuous negative pressure within the device, and 2) an outlet-free microarray layer with treelike multi-level bifurcated microchannels connecting to 4096 dead-end microwells to realize multiple loading steps until all the microwells being filled. To efficiently prevent sample evaporation at high temperatures, we use a glass slide that is pre-poured with thermosetting oil to seal the gas-permeable PDMS. Moreover, we demonstrated successful detection of single-cell methicillin-resistant Staphylococcus aureus (MRSA) with three loading steps targeting the resistance marker gene MecA via digital PCR amplification on the device.",
keywords = "Digital PCR, Microfluidic, Multi-step loading, Single-cell",
author = "Jiumei Hu and Liben Chen and Pengfei Zhang and Kuangwen Hsieh and Hui Li and Wang, {Tza Huei}",
note = "Funding Information: The authors would like to thank our funding agencies, US National Institutes of Health (grants R01AI117032, R01AI137272 and R01AI138978) for funding. Publisher Copyright: {\textcopyright} 2021 IEEE.; 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 ; Conference date: 20-06-2021 Through 25-06-2021",
year = "2021",
month = jun,
day = "20",
doi = "10.1109/Transducers50396.2021.9495676",
language = "English (US)",
series = "21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "1004--1007",
booktitle = "21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021",
}