Abstract
(Figure Presented) Donor, acceptor, and tuner: FRET-based ratiometric live-cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET-circuit-based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.
Original language | English (US) |
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Pages (from-to) | 500-502 |
Number of pages | 3 |
Journal | Angewandte Chemie - International Edition |
Volume | 47 |
Issue number | 3 |
DOIs | |
State | Published - 2008 |
Keywords
- Fluorescence
- FRET (fluorescence resonant energy transfer)
- Kinases
- Microscopy
ASJC Scopus subject areas
- General Chemistry
- General Medicine