A Tumor-suppressive Role for Trypsin in Human Cancer Progression

Keishi Yamashita, Koshi Mimori, Hiroshi Inoue, Masaki Mori, David Sidransky

Research output: Contribution to journalArticlepeer-review

70 Scopus citations


Trypsin is a serine protease family member with a potential role in cancer invasion. We investigated trypsinogen expression at the RNA level in 49 esophageal squamous cell carcinomas (ESCCs) and 72 gastric adenocarcinomas. Almost all primary ESCC tissues (95%) showed reduced expression, and 9 of 13 ESCC cell lines were silenced for trypsinogen expression. Absent expression correlated with promoter hypermethylation of trypsinogen-4 by bisulfite DNA sequence. Moreover, we detected promoter hypermethylation in 50% of primary ESCCs by methylation-specific PCR. A subset of gastric adenocarcinomas (71%) also showed reduced trypsinogen accompanied by reduction in PAR2, a G protein activated by trypsin, and a propensity to penetrate beyond the gastric wall (P = 0.001). Our results support the notion that trypsin plays a tumor-suppressive role in human carcinoma.

Original languageEnglish (US)
Pages (from-to)6575-6578
Number of pages4
JournalCancer Research
Issue number20
StatePublished - Oct 15 2003

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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