A survey of single-molecule techniques in chemical biology

Peter V. Cornish, Taekjip Ha

Research output: Contribution to journalReview articlepeer-review

131 Scopus citations


Single-molecule methods have revolutionized scientific research by rendering the investigation of once-inaccessible biological processes amenable to scientific inquiry. Several of the more established techniques will be emphasized in this Review, including single-molecule fluorescence microscopy, optical tweezers, and atomic force microscopy, which have been applied to many diverse biological processes. Serving as a taste of all the exciting research currently underway, recent examples will be discussed of translocation of RNA polymerase, myosin VI walking, protein folding, and enzyme activity. We will end by providing an assessment of what the future holds, including techniques that are currently in development.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalACS chemical biology
Issue number1
StatePublished - Jan 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine


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