@inbook{12291c76828c4b9882d6d91f70c008a5,
title = "A Split CRISPR–Cpf1 Platform for Inducible Gene Activation",
abstract = "The CRISPR–Cpf1 also known as Cas12a is an RNA-guided endonuclease similar to CRISPR–Cas9. Combining the CRISPR–Cpf1 with optogenetics technology, we have engineered photoactivatable Cpf1 (paCpf1) to precisely control the genome sequence in a spatiotemporal manner. We also identified spontaneously activated split Cpf1 and thereby developed a potent dCpf1 split activator, which has the potential to activate endogenous target genes. Here we describe a method for optogenetic endogenous genome editing using paCpf1 in mammalian cells. Furthermore, we show a method for endogenous gene activation using dCpf1 split activator in mammalian cells and mice.",
keywords = "CRISPR–Cpf1 (also known as Cas12a), crRNA, Gene activation, Genome editing, Genomic PCR, Hydrodynamic tail vein injection, In vivo gene activation, NHEJ, Optogenetics, T7E1",
author = "Takahiro Otabe and Yuta Nihongaki and Moritoshi Sato",
note = "Funding Information: We would like to thank CREST grants (JPMJCR1653) from Japan Science and Technology Agency. This work was supported by a project grant from Kanagawa Institute of Industrial Science and Technology (KISTEC) to M.S. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2724-2_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "229--240",
booktitle = "Methods in Molecular Biology",
}