@article{b0fa7ca0cb68463ab1e0068a701b72ca,
title = "A spatial map of antennal-expressed ionotropic receptors in the malaria mosquito",
abstract = "The mosquito's antenna represents its main olfactory appendage for detecting volatile chemical cues from the environment. Whole-mount fluorescence in situ hybridization of ionotropic receptors (IRs) expressed in the antennae reveals that the antenna might be divisible into proximal and distal functional domains. The number of IR-positive cells appear stereotyped within each antennal segment (flagellomere). Highly expressed odor-tuning IRs exhibit distinct co-localization patterns with the IR coreceptors Ir8a, Ir25a, and Ir76b that might predict their functional properties. Genetic knockin and in vivo functional imaging of IR41c-expressing neurons indicate both odor-induced activation and inhibition in response to select amine compounds. Targeted mutagenesis of IR41c does not abolish behavioral responses to the amine compounds. Our study provides a comprehensive map of IR-expressing neurons in the main olfactory appendage of mosquitoes. These findings show organizing principles of Anopheles IR-expressing neurons, which might underlie their functional contribution to the detection of behaviorally relevant odors.",
keywords = "CP: Neuroscience, CRISPR/Cas9, HACK, Orco, QF2, calcium imaging, olfaction, olfactory neuron, sensory biology",
author = "Raji, {Joshua I.} and Konopka, {Joanna K.} and Potter, {Christopher J.}",
note = "Funding Information: We thank Chun-Chieh Lin and Darya Task for providing the pHACK-QF2-AgU6 plasmid. We thank Ali Afify for advice on calcium imaging, as well as all the members of Potter lab for helpful discussions. This work was supported by grants from the National Institutes of Health ( NIAID R01Al137078 ) to C.J.P., the Department of Defense ( W81XWH-18-1-0732 ) to C.J.P.; an HHMI Hanna Gray fellowship to J.I.R., a Johns Hopkins Postdoctoral Accelerator Award to J.I.R., as well as a Johns Hopkins Malaria Research Institute Postdoctoral Fellowship to J.I.R.; and a Natural Science and Engineering Research Council Postdoctoral Fellowship to J.K.K.. We thank the Johns Hopkins Malaria Research Institute and Bloomberg Philanthropies for their support. Funding Information: We thank Chun-Chieh Lin and Darya Task for providing the pHACK-QF2-AgU6 plasmid. We thank Ali Afify for advice on calcium imaging, as well as all the members of Potter lab for helpful discussions. This work was supported by grants from the National Institutes of Health (NIAID R01Al137078) to C.J.P. the Department of Defense (W81XWH-18-1-0732) to C.J.P.; an HHMI Hanna Gray fellowship to J.I.R. a Johns Hopkins Postdoctoral Accelerator Award to J.I.R. as well as a Johns Hopkins Malaria Research Institute Postdoctoral Fellowship to J.I.R.; and a Natural Science and Engineering Research Council Postdoctoral Fellowship to J.K.K. We thank the Johns Hopkins Malaria Research Institute and Bloomberg Philanthropies for their support. J.I.R. performed the experiments and analyzed the data. J.I.R. and J.K.K. produced the transgenic mosquito line. J.I.R. and C.J.P. wrote the manuscript with input from J.K.K. Funding acquisition and supervision by C.J.P. The authors declare no competing interests. We support inclusive, diverse, and equitable conduct of research. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = feb,
day = "28",
doi = "10.1016/j.celrep.2023.112101",
language = "English (US)",
volume = "42",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "2",
}