A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue

Brian A. Stanley; Jennifer E. Van Eyk

doi:10.1385/1-59745-214-9:59

A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue

Brian A. Stanley, Jennifer E. Van Eyk

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

We outline a strategy for the optimization of buffer conditions for the solubilization, extraction, and isoelectric focusing (IEF) of proteins from cardiac tissue for two-dimensional gel electrophoresis (2DE). This strategy, which involves altering both the extraction and IEF buffers, allows one to ensure representation of the proteome that is as complete as possible. Initial buffer choices are given, as well as basic protocols for modifications. Although these conditions have been effectively demonstrated for human myocardium, in principle this procedure can be used for the initial screen of any new sample of tissue or cultured cells.

Original language	English (US)
Title of host publication	Cardiovascular Proteomics
Subtitle of host publication	Methods and Protocols
Editors	Fernando Vivanco
Pages	59-65
Number of pages	7
DOIs	https://doi.org/10.1385/1-59745-214-9:59
State	Published - Feb 5 2007

Publication series

Name	Methods in Molecular Biology
Volume	357
ISSN (Print)	1064-3745

Keywords

2DE
Heart muscle
IEF
Optimization
Solubilization

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1385/1-59745-214-9:59

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AB - We outline a strategy for the optimization of buffer conditions for the solubilization, extraction, and isoelectric focusing (IEF) of proteins from cardiac tissue for two-dimensional gel electrophoresis (2DE). This strategy, which involves altering both the extraction and IEF buffers, allows one to ensure representation of the proteome that is as complete as possible. Initial buffer choices are given, as well as basic protocols for modifications. Although these conditions have been effectively demonstrated for human myocardium, in principle this procedure can be used for the initial screen of any new sample of tissue or cultured cells.

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KW - Heart muscle

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KW - Optimization

KW - Solubilization

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A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue

Abstract

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Keywords

ASJC Scopus subject areas

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