TY - JOUR
T1 - A single genotype of Encephalitozoon intestinalis infects free-ranging gorillas and people sharing their habitats in Uganda
AU - Graczyk, Thaddeus K.
AU - Bosco-Nizeyi, John
AU - Da Silva, Alexandre J.
AU - Moura, Iaci N.
AU - Pieniazek, Norman J.
AU - Cranfield, Michael R.
AU - Lindquist, Alan H.
N1 - Funding Information:
Acknowledgements We thank the Uganda Wildlife Authority, Kampala-Uganda, for permission to access gorilla fecal samples; the Department of Wildlife and Animal Resource Management, Makarere University, Kampala, Uganda, for facilitating this study; and S. Doocy for her comments on the manuscript. The study was supported by two grants from the Morris Animal Foundation, Englewood, Colorado, USA (grant nos.: 98MG-11 and DO1MG-06) and the Faculty Innovation Fund, Johns Hopkins University, Baltimore, Maryland, USA (grant no.: H040-951-0189).
PY - 2002
Y1 - 2002
N2 - Microsporidian spores have been detected by Chromotrope 2R and calcofluor stains in fecal samples of three free-ranging human-habituated mountain gorillas in Uganda and in two people who share gorilla habitats. All spore isolates have been identified by PCR with species-specific primers and fluorescent in situ hybridization with a species-specific oligonucleotide probe to be Encephalitozoon intestinalis. Sequencing analyses of the full length SSUrRNA amplified from all spore isolates were identical with Enc. intestinalis SSUrRNA GenBank SIU09929. Sequences generated from a fragment containing the internal transcribed spacer of these isolates were identical to GenBank sequence Y11611, i.e., Enc. intestinalis of anthroponotic origin. A single pathogen genotype in two genetically distant but geographically united host groups indicates anthropozoonotic transmission of Enc. intestinalis. It is highly unlikely that these two identical Enc. intestinalis genotypes were acquired independently by gorillas and people; it is much more probable that one group initiated infection of the other.
AB - Microsporidian spores have been detected by Chromotrope 2R and calcofluor stains in fecal samples of three free-ranging human-habituated mountain gorillas in Uganda and in two people who share gorilla habitats. All spore isolates have been identified by PCR with species-specific primers and fluorescent in situ hybridization with a species-specific oligonucleotide probe to be Encephalitozoon intestinalis. Sequencing analyses of the full length SSUrRNA amplified from all spore isolates were identical with Enc. intestinalis SSUrRNA GenBank SIU09929. Sequences generated from a fragment containing the internal transcribed spacer of these isolates were identical to GenBank sequence Y11611, i.e., Enc. intestinalis of anthroponotic origin. A single pathogen genotype in two genetically distant but geographically united host groups indicates anthropozoonotic transmission of Enc. intestinalis. It is highly unlikely that these two identical Enc. intestinalis genotypes were acquired independently by gorillas and people; it is much more probable that one group initiated infection of the other.
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U2 - 10.1007/s00436-002-0693-5
DO - 10.1007/s00436-002-0693-5
M3 - Article
C2 - 12209334
AN - SCOPUS:0036040099
SN - 0932-0113
VL - 88
SP - 926
EP - 931
JO - Parasitology Research
JF - Parasitology Research
IS - 10
ER -