A simple, sensitive, and specific radioreceptor assay for inositol 1,4,5-trisphosphate in biological tissues

David S. Bredt, Robert J. Mourey, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

147 Scopus citations

Abstract

Inositol (1,4,5)P3 (IP3) receptors in rat cerebellar membranes have been used to develop a radioreceptor assay for endogenous IP3. The assay is sensitive, detecting as little as 1 pmole IP3 in a 0.5 ml volume. The receptor is highly selective for the 1,4,5-isomer of IP3 so that assays can employ crude tissue extracts with no purification. Chromatographic analysis and selective enzymatic hydrolysis of IP3 show that in crude tissue extracts only authentic IP3 is detected. A hundred or more samples can be assayed in a day for endogenous IP3.

Original languageEnglish (US)
Pages (from-to)976-982
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number3
DOIs
StatePublished - Mar 31 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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