A simple promoter containing two Sp1 site controls the expression of sterol-regulatory-element-binding protein 1a (SREBP-1a)

Chengkang Zhang, Dong Ju Shin, Timothy F. Osborne

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The mammalian gene for SREBP-1 (sterol-regulatory-element-binding protein 1) contains two promoters that control the production of two proteins, SREBP-1a and -1c, and each contains a unique N-terminal transcriptional activation domain, but they are otherwise identical. The relative level of each mRNA varies from tissue to tissue and they respond differently to regulatory stimuli. SREBP-1c is more abundantly expressed in liver, where its level is also regulated by insulin and liver X receptor activators, and it is also autoregulated by SREBPs. In contrast, SREBP-1a mRNA levels are relatively low and constant in different tissues and few studies have specifically analysed its pattern of expression and regulation. In the present study, we show that the promoter for SREBP-1a is contained in a very small promoter-proximal region containing two Sp1 sites. The small and relatively simple structure for its promoter provides an explanation for the low level of SREBP-1a expression. Additionally, since Sp1 has been implicated in the modest regulation of several genes by insulin, its involvement in the expression of the SREBP-1a promoter provides an explanation for the modest insulin regulation observed in animal experiments.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalBiochemical Journal
Volume386
Issue number1
DOIs
StatePublished - Feb 15 2005
Externally publishedYes

Keywords

  • Gene expression
  • Nutrient regulation
  • Promoter
  • Sp1
  • Sterol-regulatory-element- binding protein (SREBP)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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