TY - JOUR
T1 - A simple method for generation of antibodies with specificity for 1,25-dihydroxyergocalciferol and 1,25-dihydroxycholecalciferol
AU - Clemens, Thomas L.
AU - Henderson, Sheryl
AU - Meng, Xunwu
AU - Baggiolini, Enrico G.
AU - Uskokovic, Milan R.
AU - Holick, S. A.
AU - Holick, M. F.
N1 - Funding Information:
ACKNOWLEDGEMENTS This work was supported in part by NIH grants AM32324. The authors thank Catherine St.Clair and assistance in the preparation of this manuscript.
PY - 1983/11
Y1 - 1983/11
N2 - A simple method for production of antisera with high affinity and selectivity for 1α,25-dihydroxyergocalciferol and 1α,25-dihydroxycholecalciferol is described. 1α-Hydroxy-25,26,27-trisnorcholecalciferol-24-oic acid was coupled directly to bovine serum albumin. Rabbits immunized with this conjugate rapidly produced antibodies that bound 3H-1α,-25-dihydroxycholecalciferol with high affinity and demonstrated nearly equal reactivity with 1α,25-dihydroxyergoc alciferol and poor reactivity with 25-hydroxycholecalciferol; 24,25-dihydroxycholecalciferol; 25,26-dihydroxycholecalciferol; and 1β,25-dihydroxycholecalciferol. The use of one of these antisera has led to the development of a specific assay for 1α,25-dihydroxyergocalciferol and 1α,25-dihydroxycholecalciferol in human serum.
AB - A simple method for production of antisera with high affinity and selectivity for 1α,25-dihydroxyergocalciferol and 1α,25-dihydroxycholecalciferol is described. 1α-Hydroxy-25,26,27-trisnorcholecalciferol-24-oic acid was coupled directly to bovine serum albumin. Rabbits immunized with this conjugate rapidly produced antibodies that bound 3H-1α,-25-dihydroxycholecalciferol with high affinity and demonstrated nearly equal reactivity with 1α,25-dihydroxyergoc alciferol and poor reactivity with 25-hydroxycholecalciferol; 24,25-dihydroxycholecalciferol; 25,26-dihydroxycholecalciferol; and 1β,25-dihydroxycholecalciferol. The use of one of these antisera has led to the development of a specific assay for 1α,25-dihydroxyergocalciferol and 1α,25-dihydroxycholecalciferol in human serum.
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U2 - 10.1016/0039-128X(83)90114-9
DO - 10.1016/0039-128X(83)90114-9
M3 - Article
C2 - 6084881
AN - SCOPUS:0021004832
SN - 0039-128X
VL - 42
SP - 503
EP - 509
JO - Steroids
JF - Steroids
IS - 5
ER -