The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the β-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and -susceptible isolates of M. leprae.
|Original language||English (US)|
|Number of pages||5|
|Journal||International Journal of Leprosy|
|State||Published - Dec 1 1993|
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