A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

N. Honore; E. Perrani; A. Telenti; J. Grosset; S. T. Cole

A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

N. Honore, E. Perrani, A. Telenti, J. Grosset, S. T. Cole

Research output: Contribution to journal › Article › peer-review

Abstract

The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the β-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and -susceptible isolates of M. leprae.

Original language	English (US)
Pages (from-to)	600-604
Number of pages	5
Journal	International Journal of Leprosy
Volume	61
Issue number	4
State	Published - Dec 1 1993
Externally published	Yes

ASJC Scopus subject areas

Dermatology

Cite this

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title = "A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae",

abstract = "The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the β-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and -susceptible isolates of M. leprae.",

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year = "1993",

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T1 - A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

AU - Honore, N.

AU - Perrani, E.

AU - Telenti, A.

AU - Grosset, J.

AU - Cole, S. T.

PY - 1993/12/1

Y1 - 1993/12/1

N2 - The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the β-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and -susceptible isolates of M. leprae.

AB - The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the β-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and -susceptible isolates of M. leprae.

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M3 - Article

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AN - SCOPUS:0027893064

SN - 0148-916X

VL - 61

SP - 600

EP - 604

JO - International Journal of Leprosy

JF - International Journal of Leprosy

IS - 4

ER -

A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

Abstract

ASJC Scopus subject areas

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