A sensitive and specific fluorescence assay for tissue serotonin

Solomon H. Snyder, Julius Axelrod, Mark Zweig

Research output: Contribution to journalArticlepeer-review

410 Scopus citations


A sensitive and specific method for the estimation of serotonin in biological materials is described. In this method, serotonin is reacted with ninhydrin to form a product whose fluorescence is eight times more intense than the native fluorescence of serotonin in strong acid solution. With this method it is possible to measure serotonin in organs in which endogenous serotonin had not been previously detected and to study the subcellular distribution of this amine in the rat pineal and adrenal glands.

Original languageEnglish (US)
Pages (from-to)831-835
Number of pages5
JournalBiochemical Pharmacology
Issue number5
StatePublished - May 1965
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology


Dive into the research topics of 'A sensitive and specific fluorescence assay for tissue serotonin'. Together they form a unique fingerprint.

Cite this