A sensitive and specific assay for plasminogen activators

Daniel S. Smith; James Harmon; Whyte G. Owen

doi:10.1016/0049-3848(85)90099-4

A sensitive and specific assay for plasminogen activators

Daniel S. Smith, James Harmon, Whyte G. Owen

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A simple, sensitive and specific assay for plasminogen activators is described. The assay utilizes fluorescein-labeled fibrinogen or fibrin at low concentrations, and enables simultaneous evaluation of the plasminogen and fibrin dependence of the reaction, that is, discrimination of tissue-type and urokinase-type plasminogen activators, and non-specific proteolysis. Addition of antisera verify identification of the activator species. The assay reagent contains plasminogen and fluorescein-labeled fibrinogen, to which is added the specimen and then then thrombin, either at the initiation or the termination of the reaction. Supernatant fluorescence is proportional to plasminogen activator concentration. With a four-hour incubation, 1 milliunit (14 pg) of tissue (melanoma) plasminogen activator (TPA) or 2 milliunit (36 pg) of urokinase (UK) may be detected.

Original language	English (US)
Pages (from-to)	533-541
Number of pages	9
Journal	Thrombosis research
Volume	37
Issue number	4
DOIs	https://doi.org/10.1016/0049-3848(85)90099-4
State	Published - Feb 15 1985
Externally published	Yes

Keywords

fibrinolysis
plasminogen activators

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(85)90099-4

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title = "A sensitive and specific assay for plasminogen activators",

abstract = "A simple, sensitive and specific assay for plasminogen activators is described. The assay utilizes fluorescein-labeled fibrinogen or fibrin at low concentrations, and enables simultaneous evaluation of the plasminogen and fibrin dependence of the reaction, that is, discrimination of tissue-type and urokinase-type plasminogen activators, and non-specific proteolysis. Addition of antisera verify identification of the activator species. The assay reagent contains plasminogen and fluorescein-labeled fibrinogen, to which is added the specimen and then then thrombin, either at the initiation or the termination of the reaction. Supernatant fluorescence is proportional to plasminogen activator concentration. With a four-hour incubation, 1 milliunit (14 pg) of tissue (melanoma) plasminogen activator (TPA) or 2 milliunit (36 pg) of urokinase (UK) may be detected.",

keywords = "fibrinolysis, plasminogen activators",

author = "Smith, {Daniel S.} and James Harmon and Owen, {Whyte G.}",

note = "Funding Information: This work was supported by Grant HL 31223 from the National Heart Lung and Blood Institute.",

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doi = "10.1016/0049-3848(85)90099-4",

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T1 - A sensitive and specific assay for plasminogen activators

AU - Smith, Daniel S.

AU - Harmon, James

AU - Owen, Whyte G.

N1 - Funding Information: This work was supported by Grant HL 31223 from the National Heart Lung and Blood Institute.

PY - 1985/2/15

Y1 - 1985/2/15

N2 - A simple, sensitive and specific assay for plasminogen activators is described. The assay utilizes fluorescein-labeled fibrinogen or fibrin at low concentrations, and enables simultaneous evaluation of the plasminogen and fibrin dependence of the reaction, that is, discrimination of tissue-type and urokinase-type plasminogen activators, and non-specific proteolysis. Addition of antisera verify identification of the activator species. The assay reagent contains plasminogen and fluorescein-labeled fibrinogen, to which is added the specimen and then then thrombin, either at the initiation or the termination of the reaction. Supernatant fluorescence is proportional to plasminogen activator concentration. With a four-hour incubation, 1 milliunit (14 pg) of tissue (melanoma) plasminogen activator (TPA) or 2 milliunit (36 pg) of urokinase (UK) may be detected.

AB - A simple, sensitive and specific assay for plasminogen activators is described. The assay utilizes fluorescein-labeled fibrinogen or fibrin at low concentrations, and enables simultaneous evaluation of the plasminogen and fibrin dependence of the reaction, that is, discrimination of tissue-type and urokinase-type plasminogen activators, and non-specific proteolysis. Addition of antisera verify identification of the activator species. The assay reagent contains plasminogen and fluorescein-labeled fibrinogen, to which is added the specimen and then then thrombin, either at the initiation or the termination of the reaction. Supernatant fluorescence is proportional to plasminogen activator concentration. With a four-hour incubation, 1 milliunit (14 pg) of tissue (melanoma) plasminogen activator (TPA) or 2 milliunit (36 pg) of urokinase (UK) may be detected.

KW - fibrinolysis

KW - plasminogen activators

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U2 - 10.1016/0049-3848(85)90099-4

DO - 10.1016/0049-3848(85)90099-4

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AN - SCOPUS:0021951043

SN - 0049-3848

VL - 37

SP - 533

EP - 541

JO - Thrombosis research

JF - Thrombosis research

IS - 4

ER -

A sensitive and specific assay for plasminogen activators

Abstract

Keywords

ASJC Scopus subject areas

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