A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings

Pachamuthu Balakrishnan, Suniti Solomon, Janardhanan Mohanakrishnan, Anitha J. Cecelia, Nagalingeswaran Kumarasamy, Kailapuri G. Murugavel, Bhavani Venkatakrishnan, Sunil S. Solomon, Suzanne M. Crowe, Aylur K. Ganesh, Sadras P. Thyagarajan, Timothy Flanigan, Kenneth H. Mayer

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Serial measurements of absolute CD4 T-lymphocyte counts are required to initiate and gauge response to therapy and monitor disease progression. Hence, there is an urgent need to evaluate the accuracy and validity of low-cost CD4 T-cell count assays. Tripotassium EDTA blood specimens from HIV-infected individuals were studied using a novel flow cytometric assay (EasyCD4 assay; Guava Technologies, Hayward, CA) in comparison with standard flow cytometry (FACSCount; Becton Dickinson Immunocytometry Systems, San Jose, CA). The sensitivity, specificity value by EasyCD4 assay in enumerating absolute CD4 T-cell counts of less than 200 cells/μL were 95% and 100%, respectively. Bland-Altman analysis showed close agreement, with the EasyCD4 assay yielding CD4 T-cell counts a mean difference of -26 cells/μL (95% confidence interval, -96 to 44 cells/μL) higher than by flow cytometry. Our data suggest that EasyCD4 assay could be a useful alternative assay to conventional flow cytometry, may be appropriate for use in resource-limited settings.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalJournal of Acquired Immune Deficiency Syndromes
Issue number1
StatePublished - Sep 2006
Externally publishedYes


  • CD4 T-lymphocyte count
  • EasyCD4 assay
  • Flow cytometry
  • Guava technologies
  • HIV monitoring
  • Low-cost CD4
  • Resource-limited setting

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)


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