A rapid Percoll technique for the purification of human basophils

Jane A. Warner, Avner Reshef, Donald W. MacGlashan

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

We have refined a rapid single-step Percoll technique to obtain a basophil-enriched mononuclear cell fraction suitable for further purification. Greater than 75% of the total blood basophils were recovered from a blood-Percoll interface at a purity of between 5 and 23%. The contaminating cells were principally lymphocytes with a smaller (20-25%) percentage of monocytes. Further purification using the penicillin affinity column led to the recovery of between 28 and 64% of the total blood basophils at purities ranging from 52 to 92%, a substantial improvement over our earlier protocol. There was also a marked increase in the recovery of basophils from the affinity column over our previous technique, which lead to a moderate (5-10%) increase in overall recovery.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalJournal of Immunological Methods
Volume105
Issue number1
DOIs
StatePublished - Dec 4 1987

Keywords

  • Basophil purification
  • Leukocyte separation
  • Percoll

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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