A rapid manual method for CD4+ T-cell quantitation for use in developing countries

Alan Landay, John L. Ho, David Hom, Tom Russell, Robert Zwerner, Jean Gardy Minuty, Peter Kataaha, Francis Mmiro, Brooks Jackson

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Objective: To evaluate a manual method (Cytosphere) for quantifying CD4+ T-cell numbers. Design: Cross-sectional study of HIV-1-seronegative and HIV-1-seropositive individuals evaluated for absolute CD4 counts by both standardized flow cytometric measurements and manual Cytosphere technology using a hemacytometer. Setting: University research hospitals in both the United States and Africa. Patients, participants: Blood specimens from 382 patients were evaluated. These were broken down into 294 samples obtained from HIV-1-seropositive patients and 88 samples obtained from HIV-1-seronegative patients. Interventions: None. Outcome measured: Absolute CD4 cell number. Results: Evaluation of samples obtained from HIV-1 patients in both the United States and Africa demonstrated an overall correlation of the Cytosphere assay with flow cytometry of 0.912 (95% confidence interval, 0.895-0.928; P200×106/l and a 92% predictive value for correctly identifying individuals with CD4 T-cell counts 6/l. Conclusions: This assay appears to have the potential for the quantitation of CD4 cells in the limited laboratory facilities in developing countries and to have a strong correlation with standard flow cytometric technology.

Original languageEnglish (US)
Pages (from-to)1565-1568
Number of pages4
Issue number12
StatePublished - Dec 1993
Externally publishedYes


  • CD4+ T cell
  • Developing country
  • Flow cytometer
  • Hemacytometer

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy


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