@inbook{0b74bca4a730447590e93fdad1c9665e,
title = "A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis",
abstract = "Serum is a readily available source for diagnostic assays, but the identification of disease-specific serum, biomarkers has been impeded by the dominance of human serum albumin. (HSA) and immunoglobulin G (IgG) in the serum, proteome. Therefore, in order to observe lower-abundance serum proteins, removal or depletion of at least these two proteins is required. However, the depletion method needs to be inexpensive and reproducible. We describe such a protocol that combines delipidation by centrifugation, IgG removal with Protein G Sepharose{\texttrademark}, and HSA depletion with sodium chloride/ethanol precipitation. The protocol is streamlined to increase reproducibility and is compatible with many proteomie platforms, including two-dimensional gel electrophoresis, and high-performance liquid chromatography either offline or coupled online with a mass spectrometer. The reproducible depletion of lipids, IgG. and HSA permits a higher load of the remaining serum proteins, facilitating the identification of disease biomarkers.",
keywords = "Albumin, Biomarker, Depletion, Disease, HSA, IgG, Immunoglobulin, Lipid, Proteomics, Serum",
author = "Qin Fu and Bovenkamp, {Diane E.} and {Van Eyk}, {Jennifer E.}",
year = "2007",
month = feb,
day = "5",
doi = "10.1385/1-59745-214-9:365",
language = "English (US)",
isbn = "1597452149",
series = "Methods in Molecular Biology",
pages = "365--371",
editor = "Fernando Vivanco",
booktitle = "Cardiovascular Proteomics",
}