We developed a radioisotopic assay for proline oxidase in which product Δ1-pyrroline-5-carboxylate-14C is reacted with o-aminobenzaldehyde and the radioactivity trapped as the dihydroquinazolinium compound is recovered by ion-exchange chromatography. The sensitivity of this method allows the measurement of proline oxidase activity in small specimens (10 to 20 mg.) of liver.
|Number of pages
|The Journal of laboratory and clinical medicine
|Published - Feb 1975
ASJC Scopus subject areas
- Pathology and Forensic Medicine