TY - JOUR
T1 - A protonmotive force drives ATP synthesis in bacteria
AU - Maloney, P. C.
AU - Kashket, E. R.
AU - Wilson, T. H.
PY - 1974
Y1 - 1974
N2 - When cells of Streptococcus lactis or Escherichia coli were suspended in a potassium free medium, a membrane potential (negative inside) could be artificially generated by the addition of the potassium ionophore, valinomycin. In response to this inward directed protonmotive force, ATP synthesis catalyzed by the membrane bound ATPase (EC 3.6.1.3) was observed. The formation of ATP was not found in S. lactis that had been treated with the ATPase inhibitor, N,N' dicyclohexylcarbodiimide, nor was it observed in a mutant of E. coli lacking the ATPase. Inhibition of ATP synthesis in S. lactis was also observed when the membrane potential was reduced by the presence of external potassium, or when cells were first incubated with the proton conductor, carbonyl cyanide fluoromethoxy phenylhydrazone. These results are in agreement with predictions made by the chemiosmotic hypothesis of Mitchell.
AB - When cells of Streptococcus lactis or Escherichia coli were suspended in a potassium free medium, a membrane potential (negative inside) could be artificially generated by the addition of the potassium ionophore, valinomycin. In response to this inward directed protonmotive force, ATP synthesis catalyzed by the membrane bound ATPase (EC 3.6.1.3) was observed. The formation of ATP was not found in S. lactis that had been treated with the ATPase inhibitor, N,N' dicyclohexylcarbodiimide, nor was it observed in a mutant of E. coli lacking the ATPase. Inhibition of ATP synthesis in S. lactis was also observed when the membrane potential was reduced by the presence of external potassium, or when cells were first incubated with the proton conductor, carbonyl cyanide fluoromethoxy phenylhydrazone. These results are in agreement with predictions made by the chemiosmotic hypothesis of Mitchell.
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U2 - 10.1073/pnas.71.10.3896
DO - 10.1073/pnas.71.10.3896
M3 - Article
C2 - 4279406
AN - SCOPUS:0016285740
SN - 0027-8424
VL - 71
SP - 3896
EP - 3900
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -