A procedure for the bulk preparation of brain microsomes with low speed centrifugation

M. Guarnieri; A. Finnegan; O. DeMarchena

doi:10.1080/00327487608069131

A procedure for the bulk preparation of brain microsomes with low speed centrifugation

M. Guarnieri, A. Finnegan, O. DeMarchena

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Abstract

A method is described for the rapid isolation of microsomes from brains of various species. The method depends on the interaction of calcium with microsomes. The procedure is limited because calcium inhibits certain microsomal enzymes. Magnesium and manganese may be substituted for calcium. However, the substitutions decrease the reliability and efficiency of the procedure.

Original language	English (US)
Pages (from-to)	489-501
Number of pages	13
Journal	Preparative Biochemistry
Volume	6
Issue number	6
DOIs	https://doi.org/10.1080/00327487608069131
State	Published - Jan 1 1976

ASJC Scopus subject areas

Biochemistry
Genetics

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10.1080/00327487608069131

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abstract = "A method is described for the rapid isolation of microsomes from brains of various species. The method depends on the interaction of calcium with microsomes. The procedure is limited because calcium inhibits certain microsomal enzymes. Magnesium and manganese may be substituted for calcium. However, the substitutions decrease the reliability and efficiency of the procedure.",

author = "M. Guarnieri and A. Finnegan and O. DeMarchena",

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AB - A method is described for the rapid isolation of microsomes from brains of various species. The method depends on the interaction of calcium with microsomes. The procedure is limited because calcium inhibits certain microsomal enzymes. Magnesium and manganese may be substituted for calcium. However, the substitutions decrease the reliability and efficiency of the procedure.

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A procedure for the bulk preparation of brain microsomes with low speed centrifugation

Abstract

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