TY - JOUR
T1 - A photoreceptor-specific cadherin is essential for the structural integrity of the outer segment and for photoreceptor survival
AU - Rattner, Amir
AU - Smallwood, Philip M.
AU - Williams, John
AU - Cooke, Carol
AU - Savchenko, Andrey
AU - Lyubarsky, Arkady
AU - Pugh, Edward N.
AU - Nathans, Jeremy
N1 - Funding Information:
The authors thank the members of the Johns Hopkins Transgenic Core Facility for blastocyst injection, the Howard Hughes Medical Institute Biopolymers Core Facility for DNA sequencing, and Tudor Badea and King-Wai Yau for helpful comments on the manuscript. This work was supported by the Howard Hughes Medical Institute (P.M.S., J.W., and J.N.), the National Eye Institute (A.R., J.N., A.L., A.S., and E.N.P.), and Research to Prevent Blindness (E.N.P.).
PY - 2001/12/6
Y1 - 2001/12/6
N2 - A cadherin family member, prCAD, was identified in retina cDNA by subtractive hybridization and high throughput sequencing. prCAD is expressed only in retinal photoreceptors, and the prCAD protein is localized to the base of the outer segment of both rods and cones. In prCAD-/- mice, outer segments are disorganized and fragmented, and there is progressive death of photoreceptor cells. prCAD is unlikely to be involved in protein trafficking between inner and outer segments, since phototransduction proteins appear to be correctly localized and the light responses of both rods and cones are only modestly compromised in prCAD-/- mice. These experiments imply a highly specialized cell biological function for prCAD and suggest that localized adhesion activity is essential for outer segment integrity.
AB - A cadherin family member, prCAD, was identified in retina cDNA by subtractive hybridization and high throughput sequencing. prCAD is expressed only in retinal photoreceptors, and the prCAD protein is localized to the base of the outer segment of both rods and cones. In prCAD-/- mice, outer segments are disorganized and fragmented, and there is progressive death of photoreceptor cells. prCAD is unlikely to be involved in protein trafficking between inner and outer segments, since phototransduction proteins appear to be correctly localized and the light responses of both rods and cones are only modestly compromised in prCAD-/- mice. These experiments imply a highly specialized cell biological function for prCAD and suggest that localized adhesion activity is essential for outer segment integrity.
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U2 - 10.1016/S0896-6273(01)00531-1
DO - 10.1016/S0896-6273(01)00531-1
M3 - Article
C2 - 11738025
AN - SCOPUS:0035819066
SN - 0896-6273
VL - 32
SP - 775
EP - 786
JO - Neuron
JF - Neuron
IS - 5
ER -