TY - JOUR
T1 - A novel stationary phase
T2 - Capillary-channeled polymer (C-CP) fibers for HPLC separations of proteins
AU - Nelson, Dwella K.
AU - Marcus, R. Kenneth
N1 - Funding Information:
This project was supported in part by the Clemson University, National Science Foundation-sponsored Center for the Advanced Engineering Fibers and Films (CAEFF) under award number EEC-9731680. Helpful discussions with Dr. Shriram Bagrodia of the Tennessee-Eastman Corporation are greatly appreciated.
PY - 2003/10
Y1 - 2003/10
N2 - A novel stationary phase is demonstrated for the separation of proteins. Capillary-channeled polymer (C-CP) fibers provide a stationary phase that is characterized by a high surface activity (yielding strong wicking action) and drastically reduced back pressures. Columns prepared by pulling approximately 1200 50-μm diameter polypropylene C-CP fibers through stainless steel tubing with column dimensions of 4.6-mm i.d. and 306-mm length exhibit reversed-phase characteristics in the separation of the proteins. A gradient method [95:5 water-acetonitrile (ACN)/propanol (1:1) to 35:65 water-ACN/propanol] with trifluoroacetic acid added as an ion-pairing agent yields high-quality separations of superoxide dismutase, hemoglobin, hemocyanin, and myoglobin. It is believed that the C-CP fiber stationary phase holds a number of promising traits for applications in both analytical and prep-scale separations of diverse organic species, including a wide range of biomolecules.
AB - A novel stationary phase is demonstrated for the separation of proteins. Capillary-channeled polymer (C-CP) fibers provide a stationary phase that is characterized by a high surface activity (yielding strong wicking action) and drastically reduced back pressures. Columns prepared by pulling approximately 1200 50-μm diameter polypropylene C-CP fibers through stainless steel tubing with column dimensions of 4.6-mm i.d. and 306-mm length exhibit reversed-phase characteristics in the separation of the proteins. A gradient method [95:5 water-acetonitrile (ACN)/propanol (1:1) to 35:65 water-ACN/propanol] with trifluoroacetic acid added as an ion-pairing agent yields high-quality separations of superoxide dismutase, hemoglobin, hemocyanin, and myoglobin. It is believed that the C-CP fiber stationary phase holds a number of promising traits for applications in both analytical and prep-scale separations of diverse organic species, including a wide range of biomolecules.
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U2 - 10.1093/chromsci/41.9.475
DO - 10.1093/chromsci/41.9.475
M3 - Article
C2 - 14596784
AN - SCOPUS:0141782429
SN - 0021-9665
VL - 41
SP - 475
EP - 479
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
IS - 9
ER -