A novel PCR assay for quantification of HIV-1 RNA

Liang Shan; S. Alireza Rabi; Gregory M. Laird; Evelyn E. Eisele; Hao Zhang; Joseph B. Margolick; Robert F. Siliciano

doi:10.1128/JVI.00006-13

A novel PCR assay for quantification of HIV-1 RNA

Liang Shan, S. Alireza Rabi, Gregory M. Laird, Evelyn E. Eisele, Hao Zhang, Joseph B. Margolick, Robert F. Siliciano

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.

Original language	English (US)
Pages (from-to)	6521-6525
Number of pages	5
Journal	Journal of virology
Volume	87
Issue number	11
DOIs	https://doi.org/10.1128/JVI.00006-13
State	Published - Jun 2013

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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AU - Rabi, S. Alireza

AU - Laird, Gregory M.

AU - Eisele, Evelyn E.

AU - Zhang, Hao

AU - Margolick, Joseph B.

AU - Siliciano, Robert F.

PY - 2013/6

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AB - Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.

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A novel PCR assay for quantification of HIV-1 RNA

Abstract

ASJC Scopus subject areas

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