TY - JOUR
T1 - A novel K+ channel with unique localizations in mammalian brain
T2 - Molecular cloning and characterization
AU - Hwang, Paul M.
AU - Glatt, Charles E.
AU - Bredt, David S.
AU - Yellen, Gary
AU - Snyder, Solomon H.
N1 - Funding Information:
This work was supported by USPHS grant DA-00266, Research Scientist Award DA-00074 to S. H. S., training grant CM-07309 to D. S. B. and P. M. H., predoctoral fellowship MH-10017 to C. E. G., a grant from International Flavors and Fragrances, and a gift from Bristol-Myers-Squibb. We wish to thank R. R. Reed for helpful advice and M. Wang for a generous gift of rat liver genomic DNA. We also wish to thank S. Morrow and C. Papapav-IOU for prompt synthesis of oligonucleotides and N. A. Bruce for manuscript preparation. C. Y. is a Howard Hughes Medical Institute investigator.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/3
Y1 - 1992/3
N2 - Using a cDNA library prepared from circumvallate papillae of rat tongue, we have identified, cloned, and sequenced a novel K+ channel, designated cdrk. The cdrk channel appears to be a member of the Shab subfamily, most closely resembling drk1. Electrophysiologic analysis of expressed cdrk channels reveals delayed rectifier properties similar to those of drk1 channels. Localizations of cdrk mRNA in rat brain and peripheral tissues, assessed by in situ hybridization and Northern blot analysis, differ from any other reported K+ channels. In the brain cdrk mRNA is most concentrated in granule cells of the olfactory bulb and cerebellum. In peripheral tissues, mRNAs for cdrk and drk1 are reciprocally localized, indicating that the K+ channel properties contributed by mammalian Shab homologs may be important in a variety of excitable tissues.
AB - Using a cDNA library prepared from circumvallate papillae of rat tongue, we have identified, cloned, and sequenced a novel K+ channel, designated cdrk. The cdrk channel appears to be a member of the Shab subfamily, most closely resembling drk1. Electrophysiologic analysis of expressed cdrk channels reveals delayed rectifier properties similar to those of drk1 channels. Localizations of cdrk mRNA in rat brain and peripheral tissues, assessed by in situ hybridization and Northern blot analysis, differ from any other reported K+ channels. In the brain cdrk mRNA is most concentrated in granule cells of the olfactory bulb and cerebellum. In peripheral tissues, mRNAs for cdrk and drk1 are reciprocally localized, indicating that the K+ channel properties contributed by mammalian Shab homologs may be important in a variety of excitable tissues.
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U2 - 10.1016/0896-6273(92)90275-I
DO - 10.1016/0896-6273(92)90275-I
M3 - Article
C2 - 1550672
AN - SCOPUS:0026506187
SN - 0896-6273
VL - 8
SP - 473
EP - 481
JO - Neuron
JF - Neuron
IS - 3
ER -