TY - JOUR
T1 - A novel downstream regulatory element cooperates with the silencing machinery to repress EPA1 expression in candida glabrata
AU - Gallegos-García, Verónica
AU - Pan, Shih Jung
AU - Juárez-Cepeda, Jacqueline
AU - Ramírez-Zavaleta, Candy Y.
AU - Martin-del-Campo, Marcela Briones
AU - Martínez-Jiménez, Verónica
AU - Castaño, Irene
AU - Cormack, Brendan
AU - Peñas, Alejandro de Las
PY - 2012/4
Y1 - 2012/4
N2 - Candida glabrata, an opportunistic fungal pathogen, adheres to mammalian epithelial cells; adherence is mediated primarily by the Epa1 adhesin. EPA1 is a member of a large gene family of ~23 paralogues, which encode putative adhesins. In this study, we address how EPA1 transcription is regulated. Our data show that EPA1 expression is subject to two distinct negative regulatory mechanisms. EPA1 transcription is repressed by subtelomeric silencing: the Sir complex (Sir2-Sir4), Rap1, Rif1, yKu70, and yKu80 are required for full repression. Activation of EPA1 occurs immediately after dilution of stationary phase (SP) cells into fresh media; however, transcription is rapidly repressed again, limiting expression to lag phase, just as the cells exit stationary phase. This repression following lag phase requires a cis-acting regulatory negative element (NE) located in the EPA1 3'-intergenic region and is independent of telomere proximity. Bioinformatic analysis shows that there are 10 copies of the NE-like sequence in the C. glabrata genome associated with other EPA genes as well as non-EPA genes.
AB - Candida glabrata, an opportunistic fungal pathogen, adheres to mammalian epithelial cells; adherence is mediated primarily by the Epa1 adhesin. EPA1 is a member of a large gene family of ~23 paralogues, which encode putative adhesins. In this study, we address how EPA1 transcription is regulated. Our data show that EPA1 expression is subject to two distinct negative regulatory mechanisms. EPA1 transcription is repressed by subtelomeric silencing: the Sir complex (Sir2-Sir4), Rap1, Rif1, yKu70, and yKu80 are required for full repression. Activation of EPA1 occurs immediately after dilution of stationary phase (SP) cells into fresh media; however, transcription is rapidly repressed again, limiting expression to lag phase, just as the cells exit stationary phase. This repression following lag phase requires a cis-acting regulatory negative element (NE) located in the EPA1 3'-intergenic region and is independent of telomere proximity. Bioinformatic analysis shows that there are 10 copies of the NE-like sequence in the C. glabrata genome associated with other EPA genes as well as non-EPA genes.
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U2 - 10.1534/genetics.111.138099
DO - 10.1534/genetics.111.138099
M3 - Article
C2 - 22234857
AN - SCOPUS:84859571485
SN - 0016-6731
VL - 190
SP - 1285
EP - 1297
JO - Genetics
JF - Genetics
IS - 4
ER -